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sutao1979
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Сľ³æ10Äêgroupleader
- BioEPI: 1
- Ó¦Öú: 5 (Ó×¶ùÔ°)
- ¹ó±ö: 0.694
- ½ð±Ò: 3553.3
- É¢½ð: 150
- ºì»¨: 3
- Ìû×Ó: 1479
- ÔÚÏß: 64Сʱ
- ³æºÅ: 79951
- ×¢²á: 2005-07-12
- ÐÔ±ð: GG
- רҵ: Ö²Îï·ÖÀàѧ
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Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10mmols of dNTP into an acid-insoluble material in 30 minutes at 74¡æ. The reaction conditions are: 50mM Tris-HCl, (pH 9.0 at 25 ¡æ ), 50mM NaCl, 10Mm MgCl2, 200uM dATP, dCTP, dGTP and radiolabelled dTTP, and 12.5ug activated calf thymus DNA in a 50ul reaction. ¸ù¾Ýøtaqøµ¥Î»µÄ¶¨Òå¿ÉÒÔ½øÐл»Ë㣬µ«ÊÇ£¬±È½ÏÂé·³ [ Last edited by sutao1979 on 2009-3-15 at 03:06 ] |

2Â¥2009-03-15 03:02:38
aimeiaimei
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- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 6.8
- Ìû×Ó: 385
- ÔÚÏß: 96.2Сʱ
- ³æºÅ: 473224
- ×¢²á: 2007-12-05
- ÐÔ±ð: MM
- רҵ: ÉÁÖÅàÓýѧ

3Â¥2009-03-16 19:49:06














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