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The number of adherent cells to the granules of GAC was evaluated as described below. GAC granules (1 g) with the immobilized bacteria were washed three times with sterile Milli Q water and then resuspended in Tris¨CHCl 0.01 M pH 7.0 containing 0.01% Tween 80 (Sigma) and 0.1% Peptone (Sigma). The suspensions were then incubated for 10 min under constant stirring at room temperature. To measure the fraction of viable cells, the suspensions were then serially diluted in PBS 1X and plated onto TY agar plates. ÕâÆªÎÄÕÂ×îºóµÄ½á¹ûÓÃCFU / g GAC / mL ±íʾ£¬ÄÄλ´óÉñÖªµÀÔõôËã³öÀ´µÄÂ𣿼± ![]() |
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