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刘一帆

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[交流] 请大家帮忙翻译一句话

For their importance, large variety and widespread applications compared to other types of DNA biosensors, those based, for instance, on distinctive interactions of small analytes with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) and in polymerase chain reaction (PCR) amplicon detection without hybridization are the subject of hybridization based DNA biosensors justiﬁes, by itself, a new and comprehensive overview, something that this paper intends to be.

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1楼 2009-02-25 17:44:35

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刘一帆

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上下文是：The enormous amount of genetic information brought by extensive
genome sequencing has raised the need for simple, fast, cheap
and high-throughput miniaturized and mass-producible analytical
devices to attend the growing market of molecular diagnostics,
thus accomplishing the basic criteria for decentralized DNA testing.
Genome sequencing has allowed detecting, respectively, inherited
disease-causing point mutations and human pathogens through
their peculiar, specific nucleic acid sequences. Drug screening,
monitoring of differential gene expression and forensic analysis
have also benefited from the ongoing research in biosensor
technology. Such analytical devices, known as biosensors, convert
a biochemical reaction or interaction into an analytical signal
that can be further amplified, processed and recorded. Among
them, DNA biosensors consist of an immobilized DNA strand to
detect the complimentary sequence by DNA–DNA hybridization.
In a wider conception, DNA biosensors may still be conceived
to detect other analytes, with the probe molecule usually in the
form of an aptamer [1], but the study of these sensors is beyond
the scope of this review. For their importance, large variety and
widespread applications compared to other types of DNA biosensors,
those based, for instance, on distinctive interactions of small
analytes with single-stranded DNA (ssDNA) and double-stranded
DNA (dsDNA) and in polymerase chain reaction (PCR) amplicon
detection without hybridization are the subject of hybridizationbased
DNA biosensors justifies, by itself, a new and comprehensive
overview, something that this paper intends to be. Compared to
enzyme biosensors and immunosensors, there is still a scarcity
of DNA biosensors available in the market and/or under research
and development. Unlike enzymes or antibodies, DNA forms biological
recognition layers easily synthesizable, highly stable and
reusable after simple thermal melting of the DNA duplex [2].