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作者:Yang R, Liu P, Ye W.
题目: Illumina-based analysis of endophytic bacterial diversity of tree peony (Paeonia Sect. Moutan) roots and leaves
期刊:Braz J Microbiol. 2017 Oct - Dec;48(4):695-705. doi: 10.1016/j.bjm.2017.02.009
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txing: 金币+30, ★★★★★最佳答案 2017-10-18 15:57:51
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Illumina-based analysis of endophytic bacterial diversity of tree peony (Paeonia Sect. Moutan) roots and leaves.


作者:Yang, Ruixian; Liu, Ping; Ye, Wenyu




Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]



卷:48

期:4

页:695-705

DOI:10.1016/j.bjm.2017.02.009

出版年:2017 Oct - Dec (Epub 2017 Jun 02)


摘要



Diverse communities of bacteria inhabit plant tissues and those bacteria play a crucial role for plant health and growth. Tree peony (Paeonia Sect. Moutan) is known for its excellent ornamental and medicinal values as Chinese traditional plant, but little is known about its associated bacterial community under natural conditions. To examine how endophytic bacteria in tree peony vary across tissues and cultivars, PCR-based Illumina was applied to reveal the diversity of endophytic bacteria in tree peony. A total of 149,842 sequences and 21,463 operational taxonomic units (OTUs) were obtained. The OTU abundance of roots was higher than leaves across other three cultivars except for 'Kinkaku' and 'Luoyanghong'. The community was composed of five dominant groups (Proteobacteria, Firmicutes, Bacteroidetes, Acidobacteria and Actinobacteria) in all samples. Endophytic bacteria community structures had changed in leaves and roots. Sequences of Pseudomonas and Enterobacteriaceae were prevalent in root samples, whereas Succinivibrio and Acinetobacter were the dominant genus in leaf samples. Otherwise, the distribution of each dominant genus among the 5 cultivars was either varied. These findings suggested that both plant genotype and tissues contribute to the shaping of the bacterial communities associated with tree peony.
Copyright © 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.


关键词

关键词列表:Biodiversity; Endophytic bacteria; Illumina Miseq; Moutan; Paeonia Sect


作者信息

地址epartment of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang, Henan, China. Electronic address: fairy19790805@163.com.


文献信息

文献类型:Journal Article

语种:English

PubMed ID:28606427

NLM 唯一 ID:101095924

创建日期: 13 Jun 2017          修订日期: 11 Oct 2017

电子出版:02 Jun 2017

国家/地区: Brazil

ISSN:1678-4405


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