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分段设计SSR引物
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玉米基因br2的DNA ORIGIN 1 gctctgccac tctgctgagg tggggggaga ggagctcccc ctccctcctc tcccctcctc 61 gccatgtcta gcagcgaccc ggaggagatc agggcgcgcg tcgtcgttct cggttcgccc 121 catgccgacg gcggcgacga gtgggcccgg cccgagctcg aggccttcca tctgccgtct 181 cccgcccacc agcctcctgg cttcctagcc gggcaaccgg aagcagcaga gcaacccacg 241 ctccctgctc ctgctggccg cagcagcagc agcagcaaca cgcctactac atctgccggt 301 ggcggcgctg ctcctcctcc tccttcttcg cctccccctc cgccggcttc tctggagacc 361 gagcagccgc ccaatgccag gccagcctcc gccggcgcca atgacagcaa gaagcccacc 421 ccgcccgccg ccctgcgcga cctcttccgc ttcgccgacg gcctcgactg cgcgctcatg 481 ctcatcggca ccctcggcgc gctcgtccac gggtgctcgc tccccgtctt cctccgcttc 541 ttcgccgacc tcgtcgactc cttcggctcc cacgccgacg acccggacac catggtccgc 601 ctcgtcgtca agtacgcctt ctacttcctc gtcgtcggag cggcaatctg ggcatcctcg 661 tgggcaggta cgctatccct cctcctcctg ccgccccagc ttgtgtgcgt cgcgaattgg 721 cggtcaattt ggattggatg acaaatcacg tcggtcagcc aatcgccgtg gctacaaacg 781 agatgttcaa atcgttcgcc ccgctcgcaa gagatctctt gctggatgtg gaccggcgag 841 cggcagtcga cgcggatgcg gattcggtac ctggacgcgg cgctgcggca ggacgtgtcc 901 ttcttcgaca ccgacgtgcg ggcctcggac gtgatctacg ccatcaacgc ggacgccgtg 961 gtggtgcagg acgccatcag ccagaaactg ggcaacctca tccactacat ggccaccttc 1021 gtggccggct tcgtcgtggg gttcacggcc gcgtggcagc tggcgctggt cacgctggcc 1081 gtggtgccgc tcatcgccgt catcggcggg ctgagcgccg ccgcgctcgc caagctctcg 1141 tcccgcagcc aggacgcgct ctcgggcgcc agcggcatcg cggagcaggc gctcgcgcag 1201 atacggatcg tgcaggcgtt cgttggcgag gagcgcgaga tgcgggccta ctcggcggcg 1261 ctggccgtgg cgcagaggat cggctaccgc agcggcttcg ccaaggggct cggcctcggc 1321 ggcacctact tcaccgtctt ctgctgctac gggctcctgc tctggtacgg cggccacctc 1381 gtgcgcgccc agcacaccaa cggcgggctc gccatcgcca ccatgttctc cgtcatgatc 1441 ggcggactgt aaggcccacc acaccacgca ctctctcctt ctgctgtcct cggccgcccc 1501 cgtcgtcatt gctgctgacg gtatctgtgg atcgcgtgca ggcctcggca gtcggcgccg 1561 agcatggccg cgttcgccaa ggcgcgtgtg gcggctgcca agatcttccg catcatcgac 1621 cacaggccgg gcatctcctc gcgcgacggc gcggagccag agtcggtgac ggggcgggtg 1681 gagatgcggg gcgtggactt cgcgtacccg tcgcggccgg acgtccccat cctgcgcggc 1741 ttctcgctga gcgtgcccgc cgggaagacc atcgcgctgg tgggcagctc cggctccggg 1801 aagagcacgg tggtgtcgct catcgagaga ttctacgacc ccagcgcagg tatacctagt 1861 actgttacta cttttagcgc attaatctga ggatgtccag ttcgcttgct tgccaatcgc 1921 cattgccatc gcaacaacaa tacttcgcca actgccattg ctgggtagat tagtacagta 1981 gcagttagaa gaagcctcca ctgtacattg cattgccaaa caaaagtgaa ttgtgcagta 2041 actctgtacc accacattga catggaaatg aagtgaatgc ttggagcatg cagagctggc 2101 cggcctcatg ggctgctgct acctgctagc tagccaacca gaaccagcca tcctctttct 2161 tgcttttctt tttactttct ttggtcgtgg ctgtttgtgg tcatacatac attcacgcag 2221 agcagaagag ctagctaagc taggtgggtg tgcctgcaac gcgggacaaa gaaaactatt 2281 tgttgcctgg caagatgcta ctgttgccta gcacatgcct gccattgacc gactgctcag 2341 tgagaagtgg ttcagttgtg ctgttgacag tatagataga tatatatagt agccctgtag 2401 attttttttt cagacaaaaa aagaagaaga acgagatgaa gtctgcaatt cggttttggc 2461 agggcaaatc ctgctggacg ggcacgacct caggtcgctg gagctgcggt ggctgcggcg 2521 gcagatcggg ctggtgagcc aggagccggc gctgttcgcg acgagcatca gggagaacct 2581 gctgctgggg cgggacagcc agagcgcgac gctggcggag atggaggagg cggccagggt 2641 ggccaacgcc cactccttca tcatcaaact ccccgacggc tacgacacgc aggtccgtcc 2701 cgtatagcta gctcactagc tgcactgcca cttctctcgc ttgctccccc accgttgctg 2761 cctgttgctc tccaatccac ttgtcggtgt ctggaccaca cgtgctgctt gcctagctgc 2821 tccacatctg ctttccctgt ccaaccttat gcaactcact ctaatactat atcaaataca 2881 tttctagagt ttaaagctta tcttagaata aatgcatctt tagctacgag acaacctaac 2941 ttcagttgtt gttgttgttt tttttacttt ctctcttctc acaaatacta tgattacgtc 3001 tttacagcga tcttttttat tccaaaccta aaaatgcatg cactcactct aaaagcgcaa 3061 agggagcatc tttttttccc ccatcatctg cacgcagcct tttcttttcc tcatgtcacg 3121 aagggactga aggtgtgtat gcagcgtcaa gtcatccatc cgttccactc cactcactca 3181 tgcgtcgcgc actctgcgct cgtgcctgcc cggggctaaa gctttagtag ctagcctcag 3241 atcagatact gttcgtgttt gttaggccgc ggcagctgca catgagctca tgacagccgg 3301 cagcaccacc accaacgcca tggaagaggg gtcggggtcc atcacataga cataatgcct 3361 gttgtagact aggacgggag ggcaattgtt aggcgcctgt tgccatcgca tttgctgctg 3421 tgggttgcca acaagtaaca tgccaggatg ctttgctatc acgcacagga caggagaggt 3481 cctttttctc gacacaagct ctacagcctc tactaaacta gcacttgctg atgagtgcag 3541 aggatgaatg gacgatgaac atctagagtg agagagaaaa aaatgttaat aataataaaa 3601 agtagtagca ggattaagaa tcaacctggg gtacgtagga agaggtacaa tccctaggaa 3661 tctagagtat gagaagtatg ggaggagttg ggggagtgaa acggaacaaa ttccgagttg 3721 gtattttgtc gggaatgtca agttgatttt tgatcctagt gcaagcaaga attatcaatc 3781 actcagactc agcctgtctg tgtctgtcca ccccagctct tgctactcta cttactactg 3841 tgctactagt gggtagggta ggtatcttac ataaactgtt attataaact gtcatctgag 3901 aaagagagcc agtcaaaccc atgctgctgc ttattttaat cactgtcaaa tggcaggcag 3961 gcaggcagtc tggttagtta ataacatctg ggaagggttt aatcaaacca aatcaaatca 4021 gacgaaatct agaggccaca tgggatgggg ccatatgtac tgtactagca taactagcgg 4081 ctagatttta ttagaacacg gactcacact cccataacta taactgactt gatcatgatt 4141 ccttgccaag caatgctcgc atgcccatgc atgcatcatc cctggtcaaa ctcaaacact 4201 ctccaccgtc agggaataag acttattatt ttattaacaa ttcaattttt atttattaat 4261 tacgtctgga cgaggagtac tggtttattt gatgagagac atggcagtcc aagtcaaact 4321 cgtttgtctg accatggcgg tgatggccgg tgcaggttgg ggagcgcggc ctgcagctct 4381 ccggtgggca gaagcagcgc atcgccatcg cccgcgccat gctcaagaac cccgccatcc 4441 tgctgctgga cgaggccacc agcgcgctgg actccgagtc tgagaagctc gtgcaggagg 4501 cgctggaccg cttcatgatg gggcgcacca cccttggtga tcgcgcaaca ggctgtccac 4561 catccgcaaa ggccgacgtg gtggccgtgc tgcagggcgg cgccgtctcc gagatgagcg 4621 cgcacgacga gctgatggcc aagggcgaga acggcaccta cgccaagctc atccgcatgc 4681 aggagcaggc gcacgaggcg gcgctcgtca acgcccgccg cagcagcgcc aggccctcca 4741 gcgcccgcaa ctccgtcagc tcgcccatca tgacgcgcaa ctcctcctac ggccgctccc 4801 cctactcccg ccgcctctcc gacttctcca cctccgactt caccctctcc atccacgacc 4861 cgcaccacca ccaccggacc atggcggaca agcagctggc gttccgcgcc ggcgccagct 4921 ccttcctgcg cctcgccagg atgaactcgc ccgagtgggc ctacgcgctc gccggctcca 4981 tcggctccat ggtctgcggc tccttcagcg ccatcttcgc ctacatcctc agcgccgtgc 5041 tcagcgtcta ctacgcgccg gacccgcggt acatgaagcg cgagatcgca aaatactgtt 5101 acctgctcat cggcatgtcc tccgcggcgc tgctgttcaa cacggtgcag cacgtgttct 5161 gggacacggt gggcgagaac ttgaccaagc gggtgcgcga gaagatgttc gccgccgtgt 5221 tccgcaacga gatcgcctgg ttcgacgcgg acgagaacgc cagcgcgcgc gtgaccgcca 5281 ggctagcgct ggacgcccag aacgtgcgct ccgccatcgg ggaccgcatc tccgtcatcg 5341 tccagaactc ggcgctgatg ctggtggcct gcaccgcggg gttcgtcctc cagtggcgcc 5401 tcgcgctcgt gctcctcgcc gtgttcccgc tcgtcgtggg cgccaccgtg ctgcagaaga 5461 tgttcatgaa gggcttctcg ggggacctgg aggccgcgca cgccagggcc acgcagatcg 5521 cgggcgaggc cgtggccaac ctgcgcaccg tggccgcgtt caacgcggag cgcaagatca 5581 cggggctgtt cgaggccaac ctgcgcggcc cgctccggcg ctgcttctgg aaggggcaga 5641 tcgccggcag cggctacggc gtggcgcagt tcctgctgta cgcgtcctac gcgctggggc 5701 tgtggtacgc ggcgtggctg gtgaagcacg gcgtgtccga cttctcgcgc accatccgcg 5761 tgttcatggt gctgatggtg tccgcgaacg gcgccgccga gacgctgacg ctggcgccgg 5821 acttcatcaa aggcgggcgc gcgatgcggt cggtgttcga gacaatcgac cgcaagacgg 5881 aggtggagcc ccacgacgtg gacgcggcgc cggtgccgga cggcccaggg gcgaaggtgg 5941 aacttaagca cgtggacttt ttgtacccgt cgcggccgga catccaagtg ttccgcgacc 6001 tgagcctccg tgcgcgcgcc ggaaaaacgt tggcgctggt ggggccgagc gggtccggca 6061 agagctcggt cctggctctg gtgcagcggt tctacaagcc cacgtccggg cgcgtgctct 6121 tggacggcaa ggacgtgcgc aagtacaacc tgcgggcgct gcggcgcgtg gtggcggtgg 6181 taccgcagga gccgttcctg ttcgcggcga gcatccacga gaacatcgcg tacgggcgcg 6241 agggcgcgac ggaggcggag gtggtggagg cggcggcgca ggcgaacgcg caccggttca 6301 tcgcggcgct gccggagggg taccggacgc aggtgggcga gcgcggggtg cagctgtcgg 6361 gggggcagcg gcagcggatc gcgatcgcgc gcgcgctggt gaagcaggcg gccatcgtgc 6421 tgctggacga ggcgaccagc gcgctggacg ccgagtcgga gcggtgcgtg caggaggcgc 6481 tggagcgcgc ggggtccggg cgcaccacca tcgtggtggc gcaccggctg gccacggtgc 6541 gcggcgcgca caccatcgcg gtcatcgacg acggcaaggt ggcggagcag gggtcgcact 6601 cgcacctgct caagcaccat cccgacgggt gctacgcgcg gatgctgcag cttgcagcgg 6661 ctgacgggcg cggcggccgg gcccgggccg tcgtcctcgt gcaacggggc cgcgtaggac 6721 ggaatggatg gatggatggg tttggttcct cgagagattg atgggtgagg aagctgaagc 6781 tccggatcaa atggtggtac tccatgatcg caacaatgag gggaaaaaag gaaaggagaa 6841 aatacggtgg ttcatatgat tgtacaattt gacgatctgt ttgagtcggg gttttaggat 6901 gatgtaaacc ttcactcgcc ttttttttac tcttgtttct catccgcatc agtatcatct 6961 atctacatac agtgtcagag atgggaactg atcccgcatc atcatctacc tcccaaggca 7021 ccccagattg tattaatgta cttagttagc ctgttttata tatacttata agtaccaaat 7081 agcagaattt tactccttat ctgcagtagc acgaaagaaa aaaaaaaaaa gctaaacct 其 mRNA join(<64..667,812..1448,1542..1849,2463..2692,4356..>6761) /gene="pgp1" /product="PGP1" 分为8段设计SSR引物，覆盖该基因的5个外显子所有位置并相互重叠 |
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