24小时热门版块排行榜

返回列表

当前主题已经存档。

shine815

金虫 (正式写手)

应助: 1 (幼儿园)
金币: 2019.2
散金: 8
帖子: 731
在线: 42.6小时
虫号: 533433
注册: 2008-03-26
性别: GG
专业: 应用有机化学

[交流] 翻译下面几句话，多谢！

Consequently, after improvement of the wild-type HNL acting on its natural substrate, Glieder et al. tailored the enzyme for (R)-2-chloromandelonitrile production by means of in silico methods and site-directed mutagenesis. Molecular docking simulations in the active site of a homology model of PaHNL5 based on the structure of the homologous isoenzyme PaHNL1 enabled the elucidation of unfavorable steric interactions of the o-chloro substituent and amino acid residues in the catalytic center, particularly alanine 111.
Molecular modeling studies revealed a similar binding mode for (S)-2-nitro-1-phenylethanol in the catalytic center of HbHNL as it was determined experimentally for (S)-mandelonitrile, preserving all mechanistically important polar interactions with active-site residues.

回复此楼