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2498545671

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[求助] vir gene已有1人参与

我想问一下vir gene是啥?有谁知道可以告诉我一下吗?谢谢啦!

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晋鹏

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西门吹雪170: 金币+5, 鼓励回帖交流 2017-08-02 07:46:48
The Vir region on the Ti/Ri plasmid is a collection of genes whose collective function is to excise the T-DNA region of the plasmid and promote its transfer and integration into the plant genome.

The system is induced by signals produced by plants following wounding. Phenolic compounds such as acetosyringone, activate the VirA gene, which is a constitutively expressed trans-membrane protein. The activated VirA gene acts as a kinase, phosphorylating the VirG gene. In its phosphorylated form, VirG acts as an activator of transcription for the remaining Vir gene operons.

Vir D1 + D2 have endonuclease activity, and make single-stranded cuts within the left and right borders. VirE acts as a ssDNA binding protein, protecting the single strand T-DNA region during the transport phase of the process. Once in the plant cell, the complementary strand of the T-DNA is synthesised.
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2楼2017-08-01 22:37:37
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