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【答案】应助回帖
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yanghong206: 金币+200, 翻译EPI+1, ★★★★★最佳答案 2017-08-01 15:39:02
抗冻多肽 因为 具有 抑制低温储存过程中 食品 和医药 的冰晶生长、减少细胞损伤 而 保持产品品质的特点 而成为 研究热点。本项目 旨在 以食用明胶 为原材料，通过 内肽酶 切割 多肽链分子 的条件 优化控制，实现 高效 抗冻多肽 的获得，安全、无色无味 并 可重复制备。利用 超滤、色谱、小分子PAGE、质谱等生化手段 分离、鉴定 特异性抗冻多肽，表征其 抗冻活性。
Antifreeze polypeptide has become a research hotspot for its ability of maintaining the quality of food and medicine during low temperature storage via inhibiting the ice crystal growth and reducing damage injury. In this paper, an efficient antifreeze polypeptide was synthesized through cutting polypeptide chain with endopeptidase using edible gelatine as raw material. The as-prepared antifreeze polypeptide was safe, colorless and odorless, and can be prepared repeatedly with such method. The prepared antifreeze polypeptides were separated and identified by ultrafiltration, chromatography, small molecular PAGE, and mass spectrometry. Moreover, its antifreeze activity was characterized by these methods. |
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