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最近很忙，几件事情凑到一起了，有篇文章需要翻译，没有时间做，并且自己的英语水平也有限。特在此请高手帮忙翻译成中文。我浏览了哈，除专业词汇外，不是很难的， 呵呵。如果有高手能做，请先回个帖子，最好在1.17号之前弄出来。 caodong023@163.com 文题是 ：Engineering Modified Bt Toxins to Counter Insect Resistance 这个是摘要：The evolution of insect resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxinsthat are widely used in sprays and transgenic crops. Resistance to Bt toxins in some insects is linked with mutations that disrupt a toxin-binding cadherin protein. We show that susceptibility to the Bttoxin Cry1Ab was reduced by cadherin gene silencing with RNA interference in Manduca sexta,confirming cadherin’s role in Bt toxicity. Native Cry1A toxins required cadherin to form oligomers, but modified Cry1A toxins lacking one a-helix did not. The modified toxins killed cadherin-silencedM. sexta and Bt-resistant Pectinophora gossypiella that had cadherin deletion mutations. Ourfindings suggest that cadherin promotes Bt toxicity by facilitating toxin oligomerization and demonstrate that the modified Bt toxins may be useful against pests resistant to standard Bt toxins. 纳米下载 http://www.namipan.com/d/f287b47 ... e2f4b4c24845c470300 Engineering Modified Bt Toxins to.pdf |
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caitikuan
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The results suggest that in two species of Lepidoptera, cadherin receptor protein in the larval midgut mediates the toxicity of Cry1A toxins by facilitating removal of helix a-1, which promotes toxin oligomerization. 结果表明，在2种鳞翅目昆虫中，钙粘蛋白受体蛋白在介导幼虫中肠Cry1A毒素的毒性，促进消除α-1螺旋，从而促进毒素寡聚化。 The modified toxins Cry1AbMod and Cry1AcMod lacking helix α-1 formed oligomers in vitro without cadherin, whereas native Cry1Ab and Cry1Ac did not. 修改后的毒素Cry1AbMod和Cry1AcMod缺乏α - 1 螺旋，在体外形成低聚物无钙粘蛋白，而天然Cry1Ab和Cry1Ac在体外形成低聚物有钙粘蛋白。 The modified toxins killed insects with greatly reduced susceptibility to native Cry1A toxins caused by RNAi silencing of the cadherin gene or by mutations in the cadherin gene. 修改后的毒素杀死昆虫的同时大大降低天然Cry1A毒素感性，这种降低是由昆虫适应性的RNAi沉默或基因突变钙粘蛋白的基因。 These results support the pore-formation model (15) and not the signaling model, which does not include removal of helix a-1 or toxin oligomerization . 这些结果支持孔隙形成模型，而不是信号模型，其中不包括消除螺旋级或毒素低聚化。 If the results seen here with the pink bollworm extend to other lepidopterans,Cry1AbMod and Cry1AcMod could be broadly useful for countering or delaying pest resistance to Cry1A toxins. 如果在这里看到的结果，能从红铃虫扩展到其他鳞翅类昆虫 ，Cry1AbMod和Cry1AcMod大致可用于解决或推迟害虫对Cry1A毒素抗药性。 However, we do not know whether Cry1AMod toxins kill insects with mechanisms of resistance unrelated to cadherin, such as the disruption of other receptors or decreased protease activation. 然而，我们不知道Cry1AMod毒素杀死害虫的抗性机制是否和钙粘蛋白无关，如其他受体蛋白酶激活的中断或减少。 Many Bt toxins have structural topology similar to Cry1A, form oligomers, and induce pores, suggesting that they share a similar mode of action. Bt毒素和Cry1A有许多相似如拓扑结构 ，在形成低聚物及诱导空隙的过程，这表明它们具有类似的作用方式。 It remains to be determined whether, parallel to results with Cry1A toxins, other Cry toxins lacking helix a-1 can kill resistant insects that have altered receptors. Cry1A毒素与其他缺乏α-1螺旋的能够杀死改变受体且具有抗性的昆虫的Cry毒素结果是否平行还有待确定。 In addition, insects can probably evolve resistance to modified Bt toxins lacking helix a-1. 此外，昆虫可能演变出抵抗修改的缺乏α-1螺旋的Bt毒素品系。 Nonetheless, along with native Bt toxins such as Cry2 and Vip3 that have not been used as extensively as Cry1A toxins, the modified toxins broaden the options for pest control. 然而，随着野生型Bt毒素，如Cry2和Vip3还没有被用来作为广泛的Cry1A毒素，扩大了毒素的修改选择范围来控制虫害。 [ Last edited by caitikuan on 2009-1-15 at 15:35 ] |
13楼2009-01-15 14:00:36
jianwu2691
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2楼2009-01-12 17:48:35
4楼2009-01-13 13:47:25
caitikuan
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The evolution of insect resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxinsthat are widely used in sprays and transgenic crops. 抗虫性演变威胁被广泛应用在喷雾剂和转基因作物的苏云金杆菌毒素的有效性。 Resistance to Bt toxins in some insects is linked with mutations that disrupt a toxin-binding cadherin protein. 一些抗苏云金杆菌毒素的昆虫的抗性是和毒素结合钙粘蛋白的断裂相关。 We show that susceptibility to the Bttoxin Cry1Ab was reduced by cadherin gene silencing with RNA interference in Manduca sexta, confirming cadherin’s role in Bt toxicity. 在烟草天蛾体内RNA干扰使钙粘蛋白基因沉默导致苏云金杆菌毒素的Cry1Ab蛋白的敏感性降低，证明了钙粘蛋白在苏云金杆菌毒素中的作用。 Native Cry1A toxins required cadherin to form oligomers, but modified Cry1A toxins lacking one a-helix did not. 天然的Cry1A毒素需要钙粘蛋白形成寡聚体，但是改变的Cry1A缺少一个α-螺旋不能形成寡聚体。 The modified toxins killed cadherin-silenced Manduca sexta and Bt-resistant Pectinophora gossypiella that had cadherin deletion mutations. 修饰后的毒素能杀死钙粘蛋白沉默的烟草天蛾和钙粘蛋白缺失突变造成苏云金杆菌毒素抵抗的棉红铃虫。 Our findings suggest that cadherin promotes Bt toxicity by facilitating toxin oligomerization and demonstrate that the modified Bt toxins may be useful against pests resistant to standard Bt toxins. 我们的研究结果表明，钙粘蛋白通过推动毒素寡聚化作用来促进苏云金杆菌毒素的作用，还表明修饰后的苏云金杆菌毒素可能是对害虫抗药性（抗标准的苏云金杆菌毒素）有用的。 [ Last edited by caitikuan on 2009-1-15 at 10:31 ] |
5楼2009-01-13 16:41:02
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