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最近很忙，几件事情凑到一起了，有篇文章需要翻译，没有时间做，并且自己的英语水平也有限。特在此请高手帮忙翻译成中文。我浏览了哈，除专业词汇外，不是很难的， 呵呵。如果有高手能做，请先回个帖子，最好在1.17号之前弄出来。 caodong023@163.com 文题是 ：Engineering Modified Bt Toxins to Counter Insect Resistance 这个是摘要：The evolution of insect resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxinsthat are widely used in sprays and transgenic crops. Resistance to Bt toxins in some insects is linked with mutations that disrupt a toxin-binding cadherin protein. We show that susceptibility to the Bttoxin Cry1Ab was reduced by cadherin gene silencing with RNA interference in Manduca sexta,confirming cadherin’s role in Bt toxicity. Native Cry1A toxins required cadherin to form oligomers, but modified Cry1A toxins lacking one a-helix did not. The modified toxins killed cadherin-silencedM. sexta and Bt-resistant Pectinophora gossypiella that had cadherin deletion mutations. Ourfindings suggest that cadherin promotes Bt toxicity by facilitating toxin oligomerization and demonstrate that the modified Bt toxins may be useful against pests resistant to standard Bt toxins. 纳米下载 http://www.namipan.com/d/f287b47 ... e2f4b4c24845c470300 Engineering Modified Bt Toxins to.pdf |
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caitikuan
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The evolution of insect resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxinsthat are widely used in sprays and transgenic crops. 抗虫性演变威胁被广泛应用在喷雾剂和转基因作物的苏云金杆菌毒素的有效性。 Resistance to Bt toxins in some insects is linked with mutations that disrupt a toxin-binding cadherin protein. 一些抗苏云金杆菌毒素的昆虫的抗性是和毒素结合钙粘蛋白的断裂相关。 We show that susceptibility to the Bttoxin Cry1Ab was reduced by cadherin gene silencing with RNA interference in Manduca sexta, confirming cadherin’s role in Bt toxicity. 在烟草天蛾体内RNA干扰使钙粘蛋白基因沉默导致苏云金杆菌毒素的Cry1Ab蛋白的敏感性降低，证明了钙粘蛋白在苏云金杆菌毒素中的作用。 Native Cry1A toxins required cadherin to form oligomers, but modified Cry1A toxins lacking one a-helix did not. 天然的Cry1A毒素需要钙粘蛋白形成寡聚体，但是改变的Cry1A缺少一个α-螺旋不能形成寡聚体。 The modified toxins killed cadherin-silenced Manduca sexta and Bt-resistant Pectinophora gossypiella that had cadherin deletion mutations. 修饰后的毒素能杀死钙粘蛋白沉默的烟草天蛾和钙粘蛋白缺失突变造成苏云金杆菌毒素抵抗的棉红铃虫。 Our findings suggest that cadherin promotes Bt toxicity by facilitating toxin oligomerization and demonstrate that the modified Bt toxins may be useful against pests resistant to standard Bt toxins. 我们的研究结果表明，钙粘蛋白通过推动毒素寡聚化作用来促进苏云金杆菌毒素的作用，还表明修饰后的苏云金杆菌毒素可能是对害虫抗药性（抗标准的苏云金杆菌毒素）有用的。 [ Last edited by caitikuan on 2009-1-15 at 10:31 ] |
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sixmonths
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