±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 996  |  »Ø¸´: 9
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

¿ÉÒÔÂð

Òø³æ (СÓÐÃûÆø)

[½»Á÷] ÓùýNde¢ñºÍXho¢ñµÄ°ï°ïæ°¡

ÎÒ´òËãÓÃNde¢ñºÍXho¢ñÕâÁ½¸öø×ö˫øÇУ¬µ«ÊǾÝ˵ÕâÁ½¸öøøÇÐЧÂʺÍÁ¬½ÓЧÂʶ¼ºÜµÍ£¬ÇëÎÊ
1.   ÕâÁ½¸öøÄÜͬʱ×öË«ÇÐÂ𣿻¹ÊÇÒªµ¥¶ÀÇУ¿
2.   Ã¸ÇÐʱµÄøºÍÔØÌåµÄÁ¿ÊǶàÉÙ£¿Î¶ȡ¢Ê±¼äÄØ£¿
3.   ÕâÁ½¸öøÇкóvectorºÍÍâÔ´»ùÒòµÄ±ÈÀýÊǶàÉÙ£¿Ê±¼ä£¿Î¶ȣ¿


×ÜÖ®£¬ÓÐÓùýµÄ¾Í½øÀ´½²½²¾­Ñé°É£¬Ï£ÍûÄÜʹÊÔÑé½øÐÐ˳ÀûһЩ£¬Ð»Ð»À²
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

kinase

Ìú³æ (ÖøÃûдÊÖ)

ÎÒÃÇʵÑéÊÒ×î³£ÓõÄÄÚÇÐø¾ÍÊÇÕâÁ½¸ö£¬ÎÒÃÇ»ù±¾ÉÏ80%µÄ¿Ë¡¶¼ÊÇÓõÄÊÇÕâÁ½¸öø£¬¶øÇÒÎÒÃÇÊÇ»ì×ÅÓõģ¬ÓõÄÊÇNEBµÄNdeI£¬TAKARAµÄXhoI£¬bufferÒ»°ãÓÃTAKARAµÄbufferH£¬¼ÓBSA£¨BSAÓÃNEBµÄ»¹ÊÇTAKARAµÄ¶¼Ã»ÓйØÏµ£¬×¢ÒâËûÃÇÁ½¼Ò¹«Ë¾µÄ´¢ÒºÅ¨¶ÈÊDz»Ò»ÑùµÄ£©£¬50ulµÄÌåϵÀ¸÷¼Ó1ul£¬37¶È3Сʱ£¬ÍêȫûÓÐÎÊÌâ°¡¡£
5Â¥2008-12-30 19:51:02
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 10 ¸ö»Ø´ð

ÈýÁ×ËáÏÙÜÕ

Ìú¸Ëľ³æ (Ö°Òµ×÷¼Ò)

Double Digest Recommendation(s) for NdeI + XhoI:
Digest in NEBuffer 4 + BSA at 37¡ãC.


NEBµÄ½¨Òé
NEBµÄøºÜÉÙÓÐËùνµÄЧÂʵ͵ÄÎÊÌâ
¸ù¾Ýø»î¶¨Ò壬1¸öµ¥Î»µÄø1СʱÏû»¯1ug DNA
¾ßÌåµÄøÇÐÌõ¼þ¿´ËµÃ÷Êé
VectorºÍinsertµÄ±ÈÀýÄãÊÇ˵Á¬½ÓµÄʱºòÂð£¿
Ò»°ãÊÇ1£º3~1£º10£¬ÎÒϲ»¶1£º5£¨Ä¦¶û±È£©
Á¬½ÓÒ²ÓúÃÒ»µãµÄø£¬Ã»ÓÐËùνµÄÁ¬²»ÉϵÄ
²»ÒªÓÃtakaraµÄÈκι¤¾ßø£¬ÒªÂò¾ÍÂòNEBµÄ
2Â¥2008-12-29 23:01:13
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

andyhu2001

Òø³æ (СÓÐÃûÆø)

¿ÉÒÔ²éÒ»²étakaraµÄ²úÆ·ÊֲᣬÉÏÃæÓз´Ó¦Î¶ȵÈÌõ¼þ£¬Ìرð×¢ÒâÁ½¸öøµÄ·´Ó¦Î¶ÈÊÇ·ñÒ»ÖÂ
µ«ÐкÃÊ£¬ÄªÎÊǰ³Ì£¡
3Â¥2008-12-29 23:09:26
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

¿ÉÒÔÂð

Òø³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
Originally posted by kinase at 2008-12-30 19:51:
ÎÒÃÇʵÑéÊÒ×î³£ÓõÄÄÚÇÐø¾ÍÊÇÕâÁ½¸ö£¬ÎÒÃÇ»ù±¾ÉÏ80%µÄ¿Ë¡¶¼ÊÇÓõÄÊÇÕâÁ½¸öø£¬¶øÇÒÎÒÃÇÊÇ»ì×ÅÓõģ¬ÓõÄÊÇNEBµÄNdeI£¬TAKARAµÄXhoI£¬bufferÒ»°ãÓÃTAKARAµÄbufferH£¬¼ÓBSA£¨BSAÓÃNEBµÄ»¹ÊÇTAKARAµÄ¶¼Ã»ÓйØÏµ£¬×¢ ...

ллÁ˰¡£¬Õâ¸ö½â´ð±È½ÏºÃ¡£µ±È»ËùÓеĽâ´ð¶ÔÎÒ¶¼ÊÇÓаïÖúµÄ£¬·Ç³£¸Ðл¡£
6Â¥2008-12-31 13:11:46
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 351Çóµ÷¼Á +4 Âó¿Ë°¢ÀÚ 2026-03-24 4/200 2026-03-27 00:32 by wxiongid
[¿¼ÑÐ] 321Çóµ÷¼Á +6 Ymlll 2026-03-24 6/300 2026-03-26 20:50 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 327Çóµ÷¼Á +7 prayer13 2026-03-23 7/350 2026-03-26 20:48 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] »¯Ñ§¹¤³Ì085602 305·ÖÇóµ÷¼Á +17 RichLi_ 2026-03-25 17/850 2026-03-26 19:44 by plmuchong
[¿¼ÑÐ] ѧ˶274Çóµ÷¼Á +3 LiÀîÓã 2026-03-26 3/150 2026-03-26 18:32 by Ìá³ö·½·¨µÄÌá³öº
[¿¼ÑÐ] 085602 289·ÖÇóµ÷¼Á +8 WWWÎ÷Î÷¸¥Ë¹ 2026-03-24 8/400 2026-03-26 16:33 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 275Çóµ÷¼Á +9 Micky11223 2026-03-25 11/550 2026-03-26 15:54 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤¿¼Ñе÷¼Á +10 ‹üÈA 2026-03-22 10/500 2026-03-26 15:40 by zzll406
[¿¼ÑÐ] ѰÕÒµ÷¼Á +5 ¾óǿâ? 2026-03-21 8/400 2026-03-26 13:25 by 0906ljy
[¿¼ÑÐ] µ÷¼Á310 +3 ÎÂÈáµÄÍí°² 2026-03-25 4/200 2026-03-25 23:16 by peike
[¿¼ÑÐ] Ò»Ö¾Ô¸¹þ¹¤´ó£¬085400£¬320£¬Çóµ÷¼Á +4 gdlf9999 2026-03-24 4/200 2026-03-25 23:01 by boxking200
[¿¼ÑÐ] ²ÄÁÏÇóµ÷¼Á +4 .m.. 2026-03-25 4/200 2026-03-25 21:30 by peike
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤304ÇóBÇøµ÷¼Á +3 Çñgl 2026-03-25 3/150 2026-03-25 19:03 by Ainin_
[¿¼ÑÐ] 282Çóµ÷¼Á +3 wcq131415 2026-03-24 3/150 2026-03-25 12:16 by userper
[¿¼ÑÐ] Ò»Ö¾Ô¸¼ªÁÖ´óѧ²ÄÁÏÓ뻯¹¤303·ÖÇóµ÷¼Á +4 Ϊѧ666 2026-03-24 4/200 2026-03-25 11:27 by BruceLiu320
[¿¼ÑÐ] 277·ÖÇóµ÷¼Á£¬¿çµ÷²ÄÁÏ +3 ¿¼Ñе÷¼Álxh 2026-03-24 3/150 2026-03-24 13:52 by JourneyLucky
[¿¼ÑÐ] 328Çóµ÷¼Á +4 LHHL66 2026-03-23 4/200 2026-03-23 14:55 by lbsjt
[¿¼ÑÐ] 298Çóµ÷¼ÁÒ»Ö¾Ô¸211 +3 Éϰ¶6666@ 2026-03-20 3/150 2026-03-22 15:50 by ColorlessPI
[¿¼ÑÐ] Çóµ÷¼Á +5 Zhangbod 2026-03-21 7/350 2026-03-22 13:13 by Zhangbod
[¿¼ÑÐ] 22408 344·Ö Çóµ÷¼Á Ò»Ö¾Ô¸ »ªµç¼ÆËã»ú¼¼Êõ +4 solanXXX 2026-03-20 4/200 2026-03-20 23:49 by alg094825
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û