24小时热门版块排行榜

返回列表

18409207309

新虫 (著名写手)

应助: 0 (幼儿园)
金币: 1115.6
散金: 271
红花: 3
沙发: 1
帖子: 1147
在线: 94.3小时
虫号: 5655845
注册: 2017-02-20
性别: GG
专业: 化学工程及工业化学

[求助] 翻译

The direct electron transfer of myoglobin (Mb) was achieved based on the immobilization of Mb/Silver
nanoparticles (AgNPs) on glassy carbon electrode bymulti-wall carbon nanotubes (MWNTs)-chitosan(Chit) film.
The immobilized Mb displayed a pair of well-defined and reversible redox peaks with a formal potential (Eθ′
)
of ?24 mV (vs. Ag/AgCl) in 0.1 M pH 7.0 phosphate buffer solution. The apparent heterogeneous electron
transfer rate constants (ks) of Mb confined to Chit-MWNTs film was evaluated as 5.47 s?1 according to Laviron's
equation. The surface concentration (Γ?) of the electroactive Mb in the Chit-MWNTs film was estimated to be
(4.16±0.35)×10?9 mol cm?2
. Meanwhile, the catalytic ability of Mb toward the reduction of H2O2 was studied.
Its apparent Michaelis–Menten constant for H2O2 was 0.024 mM, showing a good affinity. The linear range for
H2O2 determination was from 2.5×10?5 M to 2.0×10?4 M with a detection limit of 1.02×10?6 M (S/N=3).
Moreover, the biosensor displays rapid response to H2O2 and good stability and reproducibility.
? 2011 Elsevier B.V. All rights reserved.

发自小木虫Android客户端

回复此楼