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The direct electron transfer of myoglobin (Mb) was achieved based on the immobilization of Mb/Silver nanoparticles (AgNPs) on glassy carbon electrode bymulti-wall carbon nanotubes (MWNTs)-chitosan(Chit) film. The immobilized Mb displayed a pair of well-defined and reversible redox peaks with a formal potential (Eθ′ ) of ?24 mV (vs. Ag/AgCl) in 0.1 M pH 7.0 phosphate buffer solution. The apparent heterogeneous electron transfer rate constants (ks) of Mb confined to Chit-MWNTs film was evaluated as 5.47 s?1 according to Laviron's equation. The surface concentration (Γ?) of the electroactive Mb in the Chit-MWNTs film was estimated to be (4.16±0.35)×10?9 mol cm?2 . Meanwhile, the catalytic ability of Mb toward the reduction of H2O2 was studied. Its apparent Michaelis–Menten constant for H2O2 was 0.024 mM, showing a good affinity. The linear range for H2O2 determination was from 2.5×10?5 M to 2.0×10?4 M with a detection limit of 1.02×10?6 M (S/N=3). Moreover, the biosensor displays rapid response to H2O2 and good stability and reproducibility. ? 2011 Elsevier B.V. All rights reserved. 发自小木虫Android客户端 |
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18409207309
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