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| DNA CLONING AND MOLECULAR CHARACTERIZATION OF H2A AND H4 GENES IN PEPPER |
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dengminghua: ½ð±Ò+15 2017-03-13 22:30:41
lazy½õϪ: LS-EPI+1 2017-03-13 22:57:36
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dengminghua: ½ð±Ò+15 2017-03-13 22:30:41
lazy½õϪ: LS-EPI+1 2017-03-13 22:57:36
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DNA CLONING AND MOLECULAR CHARACTERIZATION OF H2A AND H4 GENES IN PEPPER ×÷Õß eng, MH (Deng, Ming-Hua)[ 1 ] ; Zhou, H (Zhou, Hui)[ 1 ] ; Huo, JL (Huo, Jin-Long)[ 1 ] ; Lv, JH (Lv, Jun-Heng)[ 1 ] ; Wen, JF (Wen, Jin-Fen)[ 2 ] ; Zhu, HS (Zhu, Hai-Shan)[ 1 ]JOURNAL OF ANIMAL AND PLANT SCIENCES ¾í: 25 ÆÚ: 1 Ò³: 278-283 ³ö°æÄê: FEB 2015 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª The complete coding sequences (CDS) of "Yunnan Purple Pepper No. 1" (Capsicum annuum L.) H2A and H4 genes were amplified using the reverse transcriptase polymerase chain reaction based on the conserved sequence information of some Solanaceae plants and known highly homologous pepper ESTs. The nucleotide sequences analysis of these two genes revealed that pepper H2A gene encodes a protein of 142 amino acids that has high homology with the H2A-like protein of 5 species: matrimony vine, grape, alfalfa, arabidopsis and maize. Sequence analysis of the second gene revealed that the pepper H4 encodes a protein of 103 amino acids that has high homology with the proteins of 4 species: tomato, tobacco, eggplant and petunia. The tissue expression analysis indicated that the pepper H2A and H4 genes were moderately expressed in the seven tissues. Our experiment established the primary foundation for further research on these two pepper genes. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Capsicum annuum L.; H2A; H4; Gene expression profil; Bioinformatics analysis KeyWords Plus:LYSINE 56 ACETYLATION; HISTONE H2A; CORE PARTICLE; PREDICTION; EXPRESSION; PROTEINS; YEAST ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Wen, JF (ͨѶ×÷Õß) [ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ] Kunming Univ Sci & Technol, Fac Modern Agr Engn, Kunming 650224, Peoples R China. µØÖ·: [ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ] [ 1 ] Yunnan Agr Univ, Kunming 650201, Peoples R China [ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ] [ 2 ] Kunming Univ Sci & Technol, Fac Modern Agr Engn, Kunming 650224, Peoples R China µç×ÓÓʼþµØÖ·:wenjf888@163.com; shan12@sina.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National Natural Science Foundation of China 31160394 Earmarked Fund for Modern Agri-industry Technology Research System, National Department Public Benefit Research Foundation 200903025 National High Technology Research and Development Program of China 2012AA100105 Foundation of Natural Science of Yunnan Province 2009CD055 Specialized Research Fund for the Doctoral Owner of Yunnan Agricultural University ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ PAKISTAN AGRICULTURAL SCIENTISTS FORUM, UNIV VETERINARY & ANIMAL SCIENCES, LAHORE, 00000, PAKISTAN Àà±ð / ·ÖÀà Ñо¿·½Ïò:Agriculture; Life Sciences & Biomedicine - Other Topics; Veterinary Sciences Web of Science Àà±ð:Agriculture, Multidisciplinary; Biology; Veterinary Sciences ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000351716600036 ISSN: 1018-7081 ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: CE3HF Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 22 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |

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eng, MH (Deng, Ming-Hua)[ 1 ] ; Zhou, H (Zhou, Hui)[ 1 ] ; Huo, JL (Huo, Jin-Long)[ 1 ] ; Lv, JH (Lv, Jun-Heng)[ 1 ] ; Wen, JF (Wen, Jin-Fen)[ 2 ] ; Zhu, HS (Zhu, Hai-Shan)[ 1 ]