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| DNA CLONING AND MOLECULAR CHARACTERIZATION OF H2A AND H4 GENES IN PEPPER |
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dengminghua: 金币+15 2017-03-13 22:30:41
lazy锦溪: LS-EPI+1 2017-03-13 22:57:36
感谢参与，应助指数 +1
dengminghua: 金币+15 2017-03-13 22:30:41
lazy锦溪: LS-EPI+1 2017-03-13 22:57:36
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DNA CLONING AND MOLECULAR CHARACTERIZATION OF H2A AND H4 GENES IN PEPPER 作者  eng, MH (Deng, Ming-Hua)[ 1 ] ; Zhou, H (Zhou, Hui)[ 1 ] ; Huo, JL (Huo, Jin-Long)[ 1 ] ; Lv, JH (Lv, Jun-Heng)[ 1 ] ; Wen, JF (Wen, Jin-Fen)[ 2 ] ; Zhu, HS (Zhu, Hai-Shan)[ 1 ]JOURNAL OF ANIMAL AND PLANT SCIENCES 卷: 25 期: 1 页: 278-283 出版年: FEB 2015 查看期刊信息 摘要 The complete coding sequences (CDS) of "Yunnan Purple Pepper No. 1" (Capsicum annuum L.) H2A and H4 genes were amplified using the reverse transcriptase polymerase chain reaction based on the conserved sequence information of some Solanaceae plants and known highly homologous pepper ESTs. The nucleotide sequences analysis of these two genes revealed that pepper H2A gene encodes a protein of 142 amino acids that has high homology with the H2A-like protein of 5 species: matrimony vine, grape, alfalfa, arabidopsis and maize. Sequence analysis of the second gene revealed that the pepper H4 encodes a protein of 103 amino acids that has high homology with the proteins of 4 species: tomato, tobacco, eggplant and petunia. The tissue expression analysis indicated that the pepper H2A and H4 genes were moderately expressed in the seven tissues. Our experiment established the primary foundation for further research on these two pepper genes. 关键词 作者关键词:Capsicum annuum L.; H2A; H4; Gene expression profil; Bioinformatics analysis KeyWords Plus:LYSINE 56 ACETYLATION; HISTONE H2A; CORE PARTICLE; PREDICTION; EXPRESSION; PROTEINS; YEAST 作者信息 通讯作者地址: Wen, JF (通讯作者) [显示增强组织信息的名称] Kunming Univ Sci & Technol, Fac Modern Agr Engn, Kunming 650224, Peoples R China. 地址: [显示增强组织信息的名称] [ 1 ] Yunnan Agr Univ, Kunming 650201, Peoples R China [显示增强组织信息的名称] [ 2 ] Kunming Univ Sci & Technol, Fac Modern Agr Engn, Kunming 650224, Peoples R China 电子邮件地址:wenjf888@163.com; shan12@sina.com 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 31160394 Earmarked Fund for Modern Agri-industry Technology Research System, National Department Public Benefit Research Foundation 200903025 National High Technology Research and Development Program of China 2012AA100105 Foundation of Natural Science of Yunnan Province 2009CD055 Specialized Research Fund for the Doctoral Owner of Yunnan Agricultural University 查看基金资助信息 出版商 PAKISTAN AGRICULTURAL SCIENTISTS FORUM, UNIV VETERINARY & ANIMAL SCIENCES, LAHORE, 00000, PAKISTAN 类别 / 分类 研究方向:Agriculture; Life Sciences & Biomedicine - Other Topics; Veterinary Sciences Web of Science 类别:Agriculture, Multidisciplinary; Biology; Veterinary Sciences 文献信息 文献类型:Article 语种:English 入藏号: WOS:000351716600036 ISSN: 1018-7081 期刊信息 Impact Factor (影响因子): Journal Citation Reports® 其他信息 IDS 号: CE3HF Web of Science 核心合集中的 "引用的参考文献": 22 Web of Science 核心合集中的 "被引频次": 0 |
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