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[求助] Caco-2细胞与 HT29细胞区别已有1人参与

Caco-2细胞与 HT29细胞区别？有人知道吗，求指教。

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1楼 2017-02-22 21:28:42

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samule1978

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zw0123456789zw: 金币+2, ★有帮助, 谢谢 2017-02-23 18:17:24

HT29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies.

1964年，HT-29细胞系由J.Fogh 用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离。近来，已建株的培养细胞用含相同血清McCoy’s 5a培液培养。该细胞系在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。这个细胞株合成IgA, CEA, 蛋白绑定TGFβ的分泌成分和粘液素。

组织来源: 结直肠腺癌
形态: 上皮细胞

The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh.

The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. The assay requires that drug absorption rates be determined 21 days after Caco-2 cell seeding to allow for monolayer formation and cell differentiation.

Caco-2 may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug transport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.

The Caco-2 cell line

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Derived from a human colorectal carcinoma

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With remarkable morphological and biochemical similarity to the small intestinal columnar epithelium

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The apparent permeability coefficient (Papp) has been shown to correlate well with %HIA or %FA

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Extremely useful for mechanistic studies of drug absorption and as an absorption screening assay for pre-clinical drug selection.

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