24小时热门版块排行榜

返回列表

zw0123456789zw

至尊木虫 (职业作家)

Felix Baumgartner Space Jump

应助: 278 (大学生)
金币: 20607.9
散金: 286
红花: 37
帖子: 3620
在线: 1683.9小时
虫号: 313787
注册: 2007-02-24
专业: 天然药物化学

[求助] Caco-2细胞与 HT29细胞区别已有1人参与

Caco-2细胞与 HT29细胞区别？有人知道吗，求指教。

回复此楼

» 猜你喜欢

请教限项目规定已经有3人回复
拟解决的关键科学问题还要不要写已经有7人回复
存款400万可以在学校里躺平吗已经有15人回复
Materials Today Chemistry审稿周期已经有6人回复
基金委咋了？2026年的指南还没有出来？已经有10人回复
基金申报已经有6人回复
推荐一本书已经有13人回复
国自然申请面上模板最新2026版出了吗？已经有17人回复
纳米粒子粒径的测量已经有8人回复
疑惑？已经有5人回复

1楼 2017-02-22 21:28:42

已阅关注TA 给TA发消息送TA红花 TA的回帖

samule1978

铁杆木虫 (正式写手)

应助: 125 (高中生)
金币: 5452.5
红花: 18
帖子: 782
在线: 214.3小时
虫号: 4139362
注册: 2015-10-13
性别: GG
专业: 中药质量评价

【答案】应助回帖

★ ★
感谢参与，应助指数 +1
zw0123456789zw: 金币+2, ★有帮助, 谢谢 2017-02-23 18:17:24

HT29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies.

1964年，HT-29细胞系由J.Fogh 用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离。近来，已建株的培养细胞用含相同血清McCoy’s 5a培液培养。该细胞系在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。这个细胞株合成IgA, CEA, 蛋白绑定TGFβ的分泌成分和粘液素。

组织来源: 结直肠腺癌
形态: 上皮细胞

The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh.

The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. The assay requires that drug absorption rates be determined 21 days after Caco-2 cell seeding to allow for monolayer formation and cell differentiation.

Caco-2 may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug transport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.

The Caco-2 cell line

*
Derived from a human colorectal carcinoma

*
With remarkable morphological and biochemical similarity to the small intestinal columnar epithelium

*
The apparent permeability coefficient (Papp) has been shown to correlate well with %HIA or %FA

*
Extremely useful for mechanistic studies of drug absorption and as an absorption screening assay for pre-clinical drug selection.

赞一下