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【答案】应助回帖
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lxc: 金币+200, 翻译EPI+1 2017-02-04 23:27:31
以ZERAND为原料，采用酶法提取糖蛋白，在单因素实验的基础上采用响应曲面法对其提取条件进行优化，通过理化分析及UV、IR分析其性质和化学结构特征，并评价其保湿效果。结果表明：酶法提取糖蛋白的最佳工艺条件是：料液比1:30、酶解时间5h，酶解温度60 ℃，酶用量5%，酶解pH7.5，最优条件下糖蛋白的提取率为0.34%；红外吸收光谱检测表明，其具有典型的多糖特征吸收峰，且为含有α糖苷键的吡喃糖环结构；该糖蛋白具有良好的吸湿性，优于壳聚糖、丙三醇、海藻酸钠等常规保湿剂。
Glycoproteins were extracted by enzyme extraction using ZERAND as the starting matieral. The extractoin conditions were optimized based on single factor experiments following response surface methodology. The properties and chemical structures of the extracted glycoproteins were characterized by physical and chemical analysis, UV analysis and IR analysis. In addition, the moisturizing effects of the extracted glycoproteins were evaluated. The experimental results demonstrated that the optimum processing conditions to extract the glycoprotein by enzyme extraction comprised the followings: the solid-liquid ration was 1:30; the enzymolysis time period was 5h; the enzymolysis temperature was 60 ℃; the concentration of the enzyme was 5%; and the pH of enzmolysis was 7.5. The extraction yield of the glycoproteins under the optimum conditions was 0.34%. Moreover, the IR spectrum showed that the extracted glycoprotein had typical characteristic absroption peaks of polysaccride, and comprised a pyranose ring structure having an α glycosidic bond. Finally, the extracted glycoprotein had excellent moisturizing effects, which were superior to those of the conventional humectants, such as chitosan, glycol, and sodium alginate. |
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