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lxc: ½ð±Ò+200, ·ÒëEPI+1 2017-02-04 23:27:31
lxc: ½ð±Ò+200, ·ÒëEPI+1 2017-02-04 23:27:31
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ÒÔZERANDΪÔÁÏ£¬²ÉÓÃø·¨ÌáÈ¡Ìǵ°°×£¬ÔÚµ¥ÒòËØÊµÑéµÄ»ù´¡ÉϲÉÓÃÏìÓ¦ÇúÃæ·¨¶ÔÆäÌáÈ¡Ìõ¼þ½øÐÐÓÅ»¯£¬Í¨¹ýÀí»¯·ÖÎö¼°UV¡¢IR·ÖÎöÆäÐÔÖʺͻ¯Ñ§½á¹¹ÌØÕ÷£¬²¢ÆÀ¼ÛÆä±£ÊªÐ§¹û¡£½á¹û±íÃ÷£ºÃ¸·¨ÌáÈ¡Ìǵ°°×µÄ×î¼Ñ¹¤ÒÕÌõ¼þÊÇ£ºÁÏÒº±È1:30¡¢Ã¸½âʱ¼ä5h£¬Ã¸½âζÈ60 ¡æ£¬Ã¸ÓÃÁ¿5%£¬Ã¸½âpH7.5£¬×îÓÅÌõ¼þÏÂÌǵ°°×µÄÌáÈ¡ÂÊΪ0.34%£»ºìÍâÎüÊÕ¹âÆ×¼ì²â±íÃ÷£¬Æä¾ßÓеäÐ͵ĶàÌÇÌØÕ÷ÎüÊշ壬ÇÒΪº¬ÓЦÁÌÇÜÕ¼üµÄßÁà«ÌÇ»·½á¹¹£»¸ÃÌǵ°°×¾ßÓÐÁ¼ºÃµÄÎüʪÐÔ£¬ÓÅÓڿǾÛÌÇ¡¢±ûÈý´¼¡¢º£ÔåËáÄÆµÈ³£¹æ±£Êª¼Á¡£ Glycoproteins were extracted by enzyme extraction using ZERAND as the starting matieral. The extractoin conditions were optimized based on single factor experiments following response surface methodology. The properties and chemical structures of the extracted glycoproteins were characterized by physical and chemical analysis, UV analysis and IR analysis. In addition, the moisturizing effects of the extracted glycoproteins were evaluated. The experimental results demonstrated that the optimum processing conditions to extract the glycoprotein by enzyme extraction comprised the followings: the solid-liquid ration was 1:30; the enzymolysis time period was 5h; the enzymolysis temperature was 60 ¡æ; the concentration of the enzyme was 5%; and the pH of enzmolysis was 7.5. The extraction yield of the glycoproteins under the optimum conditions was 0.34%. Moreover, the IR spectrum showed that the extracted glycoprotein had typical characteristic absroption peaks of polysaccride, and comprised a pyranose ring structure having an ¦Á glycosidic bond. Finally, the extracted glycoprotein had excellent moisturizing effects, which were superior to those of the conventional humectants, such as chitosan, glycol, and sodium alginate. |
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