24小时热门版块排行榜

返回列表

zmbin

木虫 (正式写手)

应助: 7 (幼儿园)
金币: 1362.1
散金: 5728
红花: 6
帖子: 827
在线: 348.5小时
虫号: 81033
注册: 2005-07-18
专业: 生物信息学

[交流] 迷雾总会散去，NgAgo-based fabp11a gene knockdown 已有3人参与

NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish
OPEN

Jialing Qi1,*, Zhangji Dong1,*, Yunwei Shi1, Xin Wang1, Yinyin Qin1, Yongming Wang2 and Dong Liu1

1Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China
2The State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433, China
Correspondence: Dong Liu, E-mail: liudongtom@gmail.com; tom@ntu.edu.cn

*These two authors contributed equally to this work.

Top of page
Dear Editor,
A recent report of genome editing using Natronobacterium gregoryi Argonaute (NgAgo) with guide DNA (gDNA) in human cells1 prompted us to explore the utility of this protein for in vivo genetic manipulation in zebrafish (Danio rerio). Zebrafish is a model organism that offers several distinct advantages for studying genetics, developmental biology, vascular biology and disease modeling. In the last several years, loss-of-function genomic editing techniques, including zinc-finger nucleases (ZFNs)2,3, artificial transcription activator-like effector nucleases (TALENs)4,5 and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9)6,7, have been adopted for zebrafish. Recently the gDNA/NgAgo system has elicited much interest because of some unique advantages: low tolerance to guide-target mismatch, minimum off-target effects, and easy to design1. Here we investigated whether the gDNA/NgAgo system could be used to manipulate zebrafish genes in vivo using fabp11a as a test case.

发自小木虫IOS客户端

回复此楼