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迷雾总会散去，NgAgo-based fabp11a gene knockdown 已有3人参与
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NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish OPEN Jialing Qi1,*, Zhangji Dong1,*, Yunwei Shi1, Xin Wang1, Yinyin Qin1, Yongming Wang2 and Dong Liu1 1Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China 2The State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433, China Correspondence: Dong Liu, E-mail: liudongtom@gmail.com; tom@ntu.edu.cn *These two authors contributed equally to this work. Top of page Dear Editor, A recent report of genome editing using Natronobacterium gregoryi Argonaute (NgAgo) with guide DNA (gDNA) in human cells1 prompted us to explore the utility of this protein for in vivo genetic manipulation in zebrafish (Danio rerio). Zebrafish is a model organism that offers several distinct advantages for studying genetics, developmental biology, vascular biology and disease modeling. In the last several years, loss-of-function genomic editing techniques, including zinc-finger nucleases (ZFNs)2,3, artificial transcription activator-like effector nucleases (TALENs)4,5 and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9)6,7, have been adopted for zebrafish. Recently the gDNA/NgAgo system has elicited much interest because of some unique advantages: low tolerance to guide-target mismatch, minimum off-target effects, and easy to design1. Here we investigated whether the gDNA/NgAgo system could be used to manipulate zebrafish genes in vivo using fabp11a as a test case. 发自小木虫IOS客户端 |
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