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【答案】应助回帖
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icebergwu: 金币+50, 翻译EPI+1, ★★★★★最佳答案 2016-10-31 11:19:32
将物质A 粗品 用适量的 丙酮溶解，加入 适量 柱层析用硅胶，充分混合，减压旋转蒸干，让物质A粗品 完全 吸附在硅胶上。另取 一定量 硅胶 放入 层析柱中，以 丙酮 为溶剂 进行 湿法装柱,最后 将吸附了 物质A 的硅胶 置于 已经装填好的 硅胶柱上层，利用 二氯甲烷 和丙酮的 混合溶剂（添加0.1%氨水）为 洗脱剂 进行 梯度洗脱 得到 物质A纯品。
Disolve crude product of substance A with appropriate quantity of acetone, and then add appropriate amount of column chromatographic silica gel for sufficient mixing. After that, vacuum rotary evaporation was performed to make crude products fully absorbed on silica gel. Subsequently, add appropriate quantity of silica gel into the chromatographic column, and then perform wet packing method using acetone as solvent, and finally place the silica gel which has absorbed substance A on upper layer of silica gel column for gradient elution using the mixture solution of dichloromethane and acetone (with 0.1% ammonia water) as eluent, eventually resulting in pure product of substance A. |
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