24小时热门版块排行榜

返回列表

miatian1010

新虫 (初入文坛)

应助: 0 (幼儿园)
金币: 0.3
散金: 5
红花: 1
帖子: 13
在线: 5.2小时
虫号: 3289785
注册: 2014-06-24
专业: 水产生物免疫学与病害控制

[求助] 急求助：如何找到这个基因剪切体的编码蛋白质的核苷酸序列，求详细步骤，非常感谢

我想找到一个蛋白的4个剪切体的核苷酸序列，其中larp7003 和002包含内含子，又没有说明如何拼接，想找到他们的编码区，如下图及网址：

http://asia.ensembl.org/Mus_musculus/Transcript/Sequence_cDNA?db=core;g=ENSMUSG00000027968;r=3:127536714-127553349;t=ENSMUST00000195976;tl=TEm7uLIxJ6uncP7m-2216102-575424171

1 TGAAAACCAAAAAACTATGGAAGAAAGCACTAAGAGAAAAGAAGAAAAAAAGAAGCGCTC    60

                                       R
61 CCGGGTTAAACAGGTGCTTGCAGATATTGCTAAGCAGGTGGACTTCTGGTTTGGAGATGC 120

                     R
121 AAACCTTCACAAGGACAAGTTTCTGCGAGAGCAAATTGAAAAGTCTAGAGATGGATATGT 180


181 GGACATTTCTCTCCTGGTGTCTTTTAACAAAATGAAGAAGCTGACAACTGATGGGAAGCT 240

                           **************************************
241 AATAGCCAGAGCATTGAAAAGCTCATCTGTTGTAGAGGTAAAAAACTTACATTTCAAGTC 300

   *************************************************Y**********
301 ACCCACATCCAATTAACTACTGACTTTATTTAGCTTTCCTTTTTTTTTCCCCCCTCATAA 360

   ************************************************************
361 AGTTGGACTTAGAAGGGACCAGAATCAGGAGGAAAAAACCCCTAGGTGAGAGACCAAAGG 420

   *Y*****R****************************************************
421 ACGAGGAGGAACGCACGGTATATGTGGAGTTGCTCCCCAAAAATGTTACTCATAGCTGGA 480

   ************************************************************
481 TTGAGAGAGTATTTGGGAAATGTGGCAATGTGGTTTACATAAGTATTCCACATTACAAGT 540

   *************************                         R
541 CTACTGGGGATCCTAAGGGATTTGCCTTTGTGGAATTTGAAACAAAAGAGCAAGCAGCAA 600


601 AAGCCATTGAGTTTCTGAACAACCCACCAGAAGAAGCACCTAGAAAGCCTGGTATATTTC 660

                                       R R    R
661 CCAAGACAGTGAAAAACAAGCCCATCCCTTCCTTACGAGTAGCAGGTGAGTGCTTACTTA 720

      K          Y
721 GAGTGGCCAAGTAAACAATAAACTATTATGACTGTAAATAATAAAATCTTGTTACAGAAG 780


781 AGAAGAAAAAGAAGAAAAAGAAAAAAGGCAGAATAAAGAAGGAAGAGAGTGTGCAGGCCA 840

                                    S
841 AAGAGTCGGCCGTGGACAGCAGCAGCTCGGGCGTGTGTAAAGCCACGAAGAGACCAAGGA 900

                     R
901 CGGCCTCTGAGGGCTCCGAAGCAGAAACCCCGGAAGCTCCAAAGCAACCTGCAAAGAAAA 960

                                                   K
961 AGAAGAAGCGGGACAAGGTGGAAGCATCCAGCTTACCTGAAGCCAGGGCAGGGAAGAGGG 1020

                                                      W
  1021 AGAGGTGCAGCGCCGAGGACGAAGACTGCCTCCCCCCAAGGCCAAAAGCTAAGAAAAGGG 1080

                     R
  1081 CTCAGAAGGATGGCGTCGGCCAAGCAGCTTCCGAGGTCTCCAAGGAGAGCAGAGACTTAG 1140

                                    **
  1141 AATTCTGCTCTACTGAGGAGGAGAAGGAGACAGACAGAAAAGGTGACTCACTCTCAAAGG 1200


  1201 TGAAGAGGAAGCATAAGAAGAAACACAAGGAGAGACATAAGATGGGCGAGGAGGTTATAC 1260

      Y
  1261 CATTGAGAGTGCTGTCCAAGACTGAATGGATGGATTTGAAGAAAGAGTATTTGGCACTGC 1320


  1321 AAAAAGCTAGCATGGCTTCTCTAAAAAAAACAATATCTCAAATCAAATTGGAATCAGAAA 1380


  1381 TGGAAACAGAC                                                 1391