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白玉浴血木虫 (正式写手)
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[求助]
紫外可见吸收光谱，求大神帮忙解释一下！！！！
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To study and quantify the oxidation of thiols within the multilayers, 1 μm silica particles coated with five bilayers of PMASH/PVPON were used. In reduced form, the multilayers are unstable at pH 7 and rapidly disassemble when placed into a 10 mM solution of Ellman’s reagent in 1 M phosphate buffer, pH 7.5. The resulting solution has a characteristic absorption at 412 nm (Figure 2, spectrum 1), which is used to quantify the number of thiol groups by using a calibration curve obtained with standard solutions of glutathione. The addition of CaT to the particles with PMASH/PVPON multilayers at different pH (4–6) results in a decreased number of thiol groups, reflecting their oxidation into disulfide linkages. We found that the addition of 2 mole equivalents of CaT oxidized 90% of thiols within 1 min (Figure 2, spectrum 2), and a moderate excess of CaT (2 mM solution) converts the thiols into disulfides nearly quantitatively (Figure 2, spectrum 3). 请大神指教，这里的曲线1是一条校准曲线，这是校准的什么，这条曲线代表什么意义呢？曲线2和3又是怎么得来的双硫键的氧化率呢？  图片1.png |
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2楼2016-10-19 17:13:15
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