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角落里----木虫 (小有名气)
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| Enhanced lipid accumulation and biodiesel production by oleaginous Chlorella protothecoides under a structured heterotrophic-iron (II) induction strategy |
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baiyuefei
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角落里----: 金币+5, ★★★★★最佳答案 2016-09-26 16:24:28
sunshan4379: LS-EPI+1, 感谢应助！ 2016-09-26 18:11:42
感谢参与，应助指数 +1
角落里----: 金币+5, ★★★★★最佳答案 2016-09-26 16:24:28
sunshan4379: LS-EPI+1, 感谢应助！ 2016-09-26 18:11:42
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Enhanced lipid accumulation and biodiesel production by oleaginous Chlorella protothecoides under a structured heterotrophic-iron (II) induction strategy 作者:Li, YQ (Li, Yuqin)[ 1 ] ; Mu, JX (Mu, Jinxiu)[ 1 ] ; Chen, D (Chen, Di)[ 1 ] ; Xu, H (Xu, Hua)[ 1 ] ; Han, FX (Han, Fangxin)[ 1 ] WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 卷: 31 期: 5 页: 773-783 DOI: 10.1007/s11274-015-1831-4 出版年: MAY 2015 查看期刊信息 WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 出版商 SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA ISSN: 0959-3993 eISSN: 1573-0972 研究领域 Biotechnology & Applied Microbiology 摘要 A structured heterotrophic-iron (II) induction (HII) strategy was proposed to enhance lipid accumulation in oleaginous Chlorella protothecoides. C. protothecoides subjected to heterotrophic-iron (II) induction achieved a favorable lipid accumulation up to 62 % and a maximum lipid productivity of 820.17 mg/day, representing 2.78-fold and 3.64-fold increase respectively over heterotrophic cultivation alone. HII-induced cells produced significantly elevated levels of 16:0, 18:1(Delta 9), and 18:2(Delta 9,12) fatty acids (over 90 %). The lipid contents and plant lipid-like fatty acid compositions exhibit the potential of HII-induced C. protothecoides as biodiesel feedstock. Furthermore, 31 altered proteins in HII-induced algal cells were successfully identified. These differentially expressed proteins were assigned into nine molecular function categories, including carbohydrate metabolism, lipid biosynthesis, Calvin cycle, cellular respiration, photosynthesis, energy and transport, protein biosynthesis, regulate and defense, and unclassified. Analysis using the Kyoto encyclopedia of genes and genomes and gene ontology annotation showed that malic enzyme, acyltransferase, and ACP were key metabolic checkpoints found to modulate lipid accumulation in C. protothecoides. The results provided possible applications of HII cultivation strategy in other microalgal species and new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity. 关键词 作者关键词:Chlorella protothecoides; Heterotrophic-iron (II) induction; Lipid; Biodiesel; Proteomic KeyWords Plus:TRANSCRIPTION FACTOR; MICROALGAE; DEFICIENCY; VULGARIS; LIGHT; DIESEL; GROWTH; MODEL; TIME; OIL 作者信息 通讯作者地址: Li, YQ (通讯作者) Xiangtan Univ, Sch Chem Engn, Xiangtan 411105, Peoples R China. 增强组织信息的名称 Xiangtan University 地址: [ 1 ] Xiangtan Univ, Sch Chem Engn, Xiangtan 411105, Peoples R China 增强组织信息的名称 Xiangtan University 电子邮件地址:yuqinli2004@126.com 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 21306161 China Postdoctoral Science Foundation 2012M521531 2014T70775 Hunan Provincial Natural Science Foundation of China 13JJ3062 查看基金资助信息关闭基金资助信息 This work was supported by National Natural Science Foundation of China (21306161), China Postdoctoral Science Foundation Funded Project (2012M521531 and 2014T70775), and Hunan Provincial Natural Science Foundation of China (13JJ3062). 出版商 SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA 类别 / 分类 研究方向:Biotechnology & Applied Microbiology Web of Science 类别:Biotechnology & Applied Microbiology 文献信息 文献类型:Article 语种:English 入藏号: WOS:000352649200010 PubMed ID: 25724298 ISSN: 0959-3993 eISSN: 1573-0972 其他信息 IDS 号: CF6EI Web of Science 核心合集中的 "引用的参考文献": 26 Web of Science 核心合集中的 "被引频次": 1 JCR® 类别 JCR 分区 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 数据来自第 2015 版 Journal Citation Reports® |
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