24小时热门版块排行榜    

查看: 2224  |  回复: 5

20dy14

铜虫 (初入文坛)

[求助] GenBank序列提交回复CDS问题 已有1人参与

之前上传了植物its序列,然后回复如下,请问我该怎么改?
Some or all of the sequences contain incorrect annotation and/or contain
little similarity to other sequences in the database based on BLAST
searches.

Incorrect annotation may be:
- CDS or exon/intron nucleotide spans are not correct
- reading frame may be wrong and/or incorrect strand is being translated

Please review your sequences and annotation and resubmit with a corrected
file. For additional assistance, please see:

https://ncbiinsights.ncbi.nlm.ni ... -coding-region-cds/

If you are unable to determine the correct sequence and/or annotation
for your sequence(s), they can still be processed and the following
comment will be added to your records:

COMMENT  GenBank staff is unable to verify sequence and/or annotation
         provided by the submitter.

In addition, the record(s) will indicate that the data are unverified and
your sequence(s) will not be included in NCBI BLAST databases.

Please confirm that your sequence(s) should be processed as unverified.

If you need help using either Sequin or BankIt to annotate your sequence(s),
please contact GenBank Users Services at: info@ncbi.nlm.nih.gov and include
this message with your request.

Send your response to:  gb-admin@ncbi.nlm.nih.gov and/or your resubmitted
files through bankit or sequin.
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

stone2239

铁杆木虫 (著名写手)

【答案】应助回帖


感谢参与,应助指数 +1
西门吹雪170: 金币+1, 鼓励回帖交流 2016-09-18 22:32:54
ITS序列不编码蛋白,所以没有CDS区一说,也没有阅读框一说,所以你注释序列特征项feature时选错了。
2楼2016-09-18 00:56:52
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

20dy14

铜虫 (初入文坛)

引用回帖:
2楼: Originally posted by stone2239 at 2016-09-18 00:56:52
ITS序列不编码蛋白,所以没有CDS区一说,也没有阅读框一说,所以你注释序列特征项feature时选错了。

那ITS2序列应该如何上传呢?
3楼2016-09-18 16:32:35
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

20dy14

铜虫 (初入文坛)

已经解决了
4楼2016-09-18 20:16:23
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

西风实华

银虫 (正式写手)

引用回帖:
4楼: Originally posted by 20dy14 at 2016-09-18 20:16:23
已经解决了

请问你是怎么上传的啊?选择了那些选项?麻烦帮助一下啊。
5楼2016-10-30 10:22:28
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

Aimtosky

新虫 (初入文坛)

你好,我现在上传序列也出现这样的回复,请问是什么问题

发自小木虫Android客户端
6楼2018-04-03 09:37:47
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 20dy14 的主题更新
信息提示
请填处理意见