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【求助】TNBS的阳性实验是怎么回事？
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弱弱的问一下，下文出现的用三硝基苯磺酸（TNBS）的阳性实验评定氨基的成功接枝是怎么回事？ The free hydroxyl groups gave to NPs a natural, negative, surface electric potential, called Zeta potential, at neutral pH (34.3 mV). The NPs were then reacted with 3-aminopropyltriethoxysilane (APTES), which was coupled to the surface. This reaction provided positively charged NPs (referred to as amino- NPs) resulting from the presence of free amino groups at the surface. The success of the grafting procedure was assessed by Zeta potential measurements (35.8 mV at pH7) and a positive test with trinitrobenzene sulfonate (TNBS). [ Last edited by zhangwj on 2008-11-18 at 22:59 ] |
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