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鬼豆虫木虫 (正式写手)
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求助查询 Accession Number: WOS 。希望获得检索页面。文章信息如下。 文题：Transformation of prednisolone to a 20β-hydroxy prednisolonecompound by Streptomyces roseochromogenes TS79 作者：Wenquan Zhang & Li Cui & Mengyao Wu &Rongqing Zhang & Liping Xie & Hongzhong Wang 期刊号：Appl Microbiol Biotechnol (2011) 92:727–735 DOI 10.1007/s00253-011-3382-4 |
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baiyuefei
版主 (文学泰斗)
风雪
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【答案】应助回帖
感谢参与，应助指数 +1
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Transformation of prednisolone to a 20 beta-hydroxy prednisolone compound by Streptomyces roseochromogenes TS79 作者:Zhang, WQ (Zhang, Wenquan)[ 1,2 ] ; Cui, L (Cui, Li)[ 3 ] ; Wu, MY (Wu, Mengyao)[ 3 ] ; Zhang, RQ (Zhang, Rongqing)[ 1,2 ] ; Xie, LP (Xie, Liping)[ 1,2 ] ; Wang, HZ (Wang, Hongzhong)[ 1,2 ] APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 卷: 92 期: 4 页: 727-735 DOI: 10.1007/s00253-011-3382-4 出版年: NOV 2011 查看期刊信息 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 出版商 SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA ISSN: 0175-7598 研究领域 Biotechnology & Applied Microbiology 摘要 Prednisolone represents an important compound in pharmaceutical preparations. To obtain more bioactive prednisolone derivatives, the microbial transformation of prednisolone was carried out. The steroid products were assigned by an interpretation of their spectral data using mass spectrometry and proton nuclear magnetic resonance ((1)H NMR) analyses. The product was assigned the chemical structure of 11 beta, 17 alpha, 20 beta, 21-tetrahydroxypregna-1,4-diene-3-one (named as 20 beta-hydroxy prednisolone). The conversion of prednisolone to 20 beta-hydroxy prednisolone by Streptomyces roseochromogenes TS79 was different from a previous study on the microbial transformation of steroid by this organism, which usually generates a 16 alpha-hydroxy steroid product. The different reaction parameters for maximum conversion of prednisolone were optimized. The analysis revealed that the optimum values of the tested variables were 7.5 mg/ml prednisolone dissolved in DMSO and added to the 24 h pre-culture fermentation culture containing 0.05% MgSO(4) and incubated for 24 h. A conversion of 95.1% of prednisolone was observed, which has the potential to be used in industrial production. 关键词 作者关键词:Streptomyces roseochromogenes TS79; C20 beta-hydroxy prednisolone; Microbial transformation; Hydroxylation KeyWords Plus:SPINAL-CORD-INJURY; MICROBIAL TRANSFORMATION; ESCHERICHIA-COLI; HETEROLOGOUS EXPRESSION; CUNNINGHAMELLA-ELEGANS; ACREMONIUM-STRICTUM; BIOTRANSFORMATION; CYTOCHROME-P450; STEROIDS; CONVERSION 作者信息 通讯作者地址: Wang, HZ (通讯作者) Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China. 增强组织信息的名称 Tsinghua University 地址: [ 1 ] Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China 增强组织信息的名称 Tsinghua University [ 2 ] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 增强组织信息的名称 Tsinghua University [ 3 ] Dalian Polytech Univ, Sch Chem Engn & Mat, Dalian 116034, Peoples R China 增强组织信息的名称 Dalian Polytechnic University 电子邮件地址:hzwang@tsinghua.edu.cn 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 20876088 查看基金资助信息关闭基金资助信息 This work was supported by grants from National Natural Science Foundation of China (project no. 20876088). 出版商 SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA 类别 / 分类 研究方向:Biotechnology & Applied Microbiology Web of Science 类别:Biotechnology & Applied Microbiology 文献信息 文献类型:Article 语种:English 入藏号: WOS:000296779200007 PubMed ID: 21670977 ISSN: 0175-7598 其他信息 IDS 号: 844VU Web of Science 核心合集中的 "引用的参考文献": 35 Web of Science 核心合集中的 "被引频次": 6 JCR® 类别 JCR 分区 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q2 数据来自第 2015 版 Journal Citation Reports® |
2楼2016-08-12 20:20:03
baiyuefei
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- 应助: 4642 (副教授)
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- 散金: 11616
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- 专业: 合成药物化学
- 管辖: 有机交流
【答案】应助回帖
★ ★ ★ ★ ★
鬼豆虫: 金币+5, ★★★★★最佳答案 2016-08-12 20:55:30
sunshan4379: LS-EPI+1, 感谢应助！ 2016-08-12 21:16:32
鬼豆虫: 金币+5, ★★★★★最佳答案 2016-08-12 20:55:30
sunshan4379: LS-EPI+1, 感谢应助！ 2016-08-12 21:16:32
3楼2016-08-12 20:20:11