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[求助] 一般怎么找到这个Tub-tag的啊

Abstract: A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported. Recombinant
tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles
to proteins containing a short tubulin-derived recognition sequence (Tub-tag).
This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for
applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP,and ubiquitin.

这是一个论文的摘要,作者在文章提到TTL酶可以特异识别一个14个氨基酸组成的多肽,也就是这个Tub-tag。我想了解的是:一般怎么找到这个Tub-tag的啊,用什么方法啊。

1.how can you find the fourteen amino sequence, which can be served as tubulin-derived recognition
sequence (Tub-tag)?

2. what methods will you need to find the sequence?

3.or what is the general process to find the sequence?

求大神解答。

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  • 附件 1 : Schumacher_et_al-2015-Angewandte_Chemie_International_Edition.pdf
  • 2016-07-24 11:24:51, 3.26 M

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