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请问怎样查询 Accession Number: WOS ？ 文题：Transformation of prednisolone to a 20β-hydroxy prednisolonecompound by Streptomyces roseochromogenes TS79 作者：Wenquan Zhang & Li Cui & Mengyao Wu &Rongqing Zhang & Liping Xie & Hongzhong Wang 期刊号：Appl Microbiol Biotechnol (2011) 92:727–735 DOI 10.1007/s00253-011-3382-4 |
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baiyuefei
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鬼豆虫: 金币+5, ★★★★★最佳答案 2016-07-21 12:39:17
lazy锦溪: LS-EPI+1, 感谢应助！ 2016-07-21 13:06:10
感谢参与，应助指数 +1
鬼豆虫: 金币+5, ★★★★★最佳答案 2016-07-21 12:39:17
lazy锦溪: LS-EPI+1, 感谢应助！ 2016-07-21 13:06:10
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https://apps.webofknowledge.com/ ... age=1&doc=1 Transformation of prednisolone to a 20 beta-hydroxy prednisolone compound by Streptomyces roseochromogenes TS79 ×÷??:Zhang, WQ (Zhang, Wenquan)[ 1,2 ] ; Cui, L (Cui, Li)[ 3 ] ; Wu, MY (Wu, Mengyao)[ 3 ] ; Zhang, RQ (Zhang, Rongqing)[ 1,2 ] ; Xie, LP (Xie, Liping)[ 1,2 ] ; Wang, HZ (Wang, Hongzhong)[ 1,2 ] APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ?í: 92 ??: 4 ??: 727-735 DOI: 10.1007/s00253-011-3382-4 ??°??ê: NOV 2011 ?é?????????? APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ??°??? SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA ISSN: 0175-7598 ?????ì?ò Biotechnology & Applied Microbiology ???? Prednisolone represents an important compound in pharmaceutical preparations. To obtain more bioactive prednisolone derivatives, the microbial transformation of prednisolone was carried out. The steroid products were assigned by an interpretation of their spectral data using mass spectrometry and proton nuclear magnetic resonance ((1)H NMR) analyses. The product was assigned the chemical structure of 11 beta, 17 alpha, 20 beta, 21-tetrahydroxypregna-1,4-diene-3-one (named as 20 beta-hydroxy prednisolone). The conversion of prednisolone to 20 beta-hydroxy prednisolone by Streptomyces roseochromogenes TS79 was different from a previous study on the microbial transformation of steroid by this organism, which usually generates a 16 alpha-hydroxy steroid product. The different reaction parameters for maximum conversion of prednisolone were optimized. The analysis revealed that the optimum values of the tested variables were 7.5 mg/ml prednisolone dissolved in DMSO and added to the 24 h pre-culture fermentation culture containing 0.05% MgSO(4) and incubated for 24 h. A conversion of 95.1% of prednisolone was observed, which has the potential to be used in industrial production. ???ü?? ×÷?????ü??:Streptomyces roseochromogenes TS79; C20 beta-hydroxy prednisolone; Microbial transformation; Hydroxylation KeyWords Plus:SPINAL-CORD-INJURY; MICROBIAL TRANSFORMATION; ESCHERICHIA-COLI; HETEROLOGOUS EXPRESSION; CUNNINGHAMELLA-ELEGANS; ACREMONIUM-STRICTUM; BIOTRANSFORMATION; CYTOCHROME-P450; STEROIDS; CONVERSION ×÷?????? ?¨??×÷?????·: Wang, HZ (?¨??×÷??) Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China. ????×é???????????? Tsinghua University ???·: [ 1 ] Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China ????×é???????????? Tsinghua University [ 2 ] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China ????×é???????????? Tsinghua University [ 3 ] Dalian Polytech Univ, Sch Chem Engn & Mat, Dalian 116034, Peoples R China ????×é???????????? Dalian Polytechnic University ??×????????·:hzwang@tsinghua.edu.cn ?ù??×??ú???? ?ù??×??ú?ú?? ???¨?? National Natural Science Foundation of China 20876088 ?é???ù??×??ú??????±??ù??×??ú???? This work was supported by grants from National Natural Science Foundation of China (project no. 20876088). ??°??? SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA ?à±? / ·??à ????·??ò:Biotechnology & Applied Microbiology Web of Science ?à±?:Biotechnology & Applied Microbiology ???×???? ???×?à??:Article ????:English ??????: WOS:000296779200007 PubMed ID: 21670977 ISSN: 0175-7598 ???????? IDS ??: 844VU Web of Science ???????????? "?????????????×": 35 Web of Science ???????????? "±???????": 6 JCR® ?à±? JCR ·??? BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q2 ??????×??? 2015 °? Journal Citation Reports® |
2楼2016-07-21 12:18:05
