[½»Á÷] ÓÐÈËÔÚ0¡æÏÂÓÃstober·¨ÔÚ´ÅÐÔ΢Çò±íÃæÓÃTEOS°ü¹ü¶þÑõ»¯¹èµÄÂ𣿠ÒÑÓÐ3È˲ÎÓë

Afterward, the preparation of Fe3O4@SiO2@NH2 core/shell microspheres was carried out by the Stober process27 with minor changes. The synthesized magnetic nanoparticles (0.040 g) were homogeneously dispersed in a mixture containing ethanol (128 mL), deionized water (36 mL), and 28% (wt) concentrated ammonia aqueous solution (NH3¡¤H2O), followed by the addition of 0.20 g of tetraethyl orthosilicate (TEOS). Then, the reaction was allowed to proceed at 0 ¡ãC for 1 hunder sonication to get the silica embedded magnetic nanoparticles. Finally, 0.020 g of 3-aminopropyl-triethoxysilane (APTES) was added to the above mixture, and the reaction was performed for another 20 min under sonication. The harvested Fe3O4@SiO2@NH2 microspheres were collected with a magnet and washed repeatedly with ethanol and water to effectively remove nonmagnetic byproducts and residual reagents. The final products were dried under vacuum at 50 ¡ãC for further use. ¿´¼û Analytical ChemistryÉÏ ÔÚ0¡æϺϳɵģ¬ÓÐÊÔ¹ýÕâÑùºÏ³ÉµÄͬѧÂ𣿠ÎÄÕÂÌâÄ¿ÊÇ£ºAn Accessible Protocol for Solid-Phase Extraction of N‑Linked Glycopeptides through Reductive Amination by AmineFunctionalized Magnetic Nanoparticles

»Ø¸´´ËÂ¥