|
|
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
paper(lazy锦溪代发): 金币+20, 协助结帖，感谢应助！ 2016-05-12 09:09:56
lazy锦溪: LS-EPI+1, 感谢应助！ 2016-05-12 09:10:01
Folliculin Deficient Renal Cancer Cells Show Higher Radiosensitivity through Autophagic Cell Death
作者:Zhang, Q (Zhang, Qi)[ 1,2 ] ; Si, SH (Si, Shuhui)[ 2 ] ; Schoen, S (Schoen, Sue)[ 2 ] ; Jin, XB (Jin, Xun-Bo)[ 1 ] ; Chen, JD (Chen, Jindong)[ 2 ] ; Wu, G (Wu, Guan)[ 2,3,4 ]
JOURNAL OF UROLOGY
卷: 191
期: 6
页: 1880-1888
DOI: 10.1016/j.juro.2014.01.001
出版年: JUN 2014
查看期刊信息
JOURNAL OF UROLOGY
出版商 ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
ISSN: 0022-5347
eISSN: 1527-3792
研究领域 Urology & Nephrology
摘要
Purpose: Mutations in the FLCN gene are responsible for fibrofolliculoma, pulmonary and renal cysts, and renal cell carcinoma in patients with Birt-Hogg-Dube syndrome. To explore therapeutic approaches to renal cell carcinoma in patients with Birt-Hogg-Dube syndrome we investigated the anticancer effects of irradiation on folliculin deficient renal cancer cells.
Materials and Methods: Folliculin deficient (UOK257 and ACHN-5968) and folliculin expressing (UOK257-2 and ACHN-sc) cell lines were used in this study. Clonogenic assays were used to determine the radiosensitivity of folliculin deficient and expressing renal cell carcinoma cells. Apoptosis was detected in these cells by DAPI and TUNEL assays after irradiation. Monodansylcadaverine analysis, GFP-LC3 assay and Western blot were performed to monitor the autophagic process.
Results: Folliculin deficient cells were more sensitive to irradiation than their folliculin expressing counterparts. The enhanced effects of irradiation on folliculin deficient cells were mediated by increased autophagy but not by apoptosis. An increased Beclin 1 protein level and an activated mitogen-activated protein kinase pathway were identified as the key regulators of increased autophagy in these folliculin deficient cells. Inhibiting autophagy with 3-methyladenine or beclin 1 siRNA obviously increased radioresistance in folliculin deficient cells. Moreover, irradiation combined with autophagic inducer rapamycin significantly increased autophagy and radiosensitivity in folliculin deficient renal cell carcinoma cells.
Conclusions: Findings suggest that folliculin deficient renal cell carcinoma cells are highly sensitive to irradiation due to increased autophagic cell death, unlike other types of renal cell carcinoma. Irradiation plus autophagy inducers, eg rapamycin, might be a potentially more effective therapeutic approach to folliculin deficient renal cell carcinoma.
关键词
作者关键词:kidney; carcinoma; renal cell; Birt-Hogg-Dube syndrome; estrone; autophagy
KeyWords Plus:HOGG-DUBE-SYNDROME; MALIGNANT GLIOMA-CELLS; PROSTATE-CANCER; INDUCTION; CARCINOMA; APOPTOSIS; INHIBITION; PATHWAY; PROTEIN; TUMORS
作者信息
通讯作者地址: Chen, JD (通讯作者) Univ Rochester, Med Ctr, Dept Urol, 601 Elmwood Ave,Box 656, Rochester, NY 14642 USA.
增强组织信息的名称
University of Rochester
地址: [ 1 ] Shandong Univ, Prov Hosp, Minimally Invas Urol Ctr, Jinan 250100, Peoples R China
增强组织信息的名称
Shandong University
[ 2 ] Univ Rochester, Med Ctr, Dept Urol, Rochester, NY 14642 USA
增强组织信息的名称
University of Rochester
[ 3 ] Univ Rochester, Med Ctr, Dept Pathol, Rochester, NY 14642 USA
增强组织信息的名称
University of Rochester
[ 4 ] Univ Rochester, Med Ctr, Wilmot Canc Ctr, Rochester, NY 14642 USA
增强组织信息的名称
University of Rochester
电子邮件地址:jindong_chen@urmc.rochester.edu; guan_wu@urmc.rochester.edu
基金资助致谢
基金资助机构 授权号
Department of Urology, University of Rochester Medical Center
查看基金资助信息关闭基金资助信息
Supported by the Department of Urology, University of Rochester Medical Center.
出版商
ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
类别 / 分类
研究方向:Urology & Nephrology
Web of Science 类别:Urology & Nephrology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000336531100100
PubMed ID: 24434776
ISSN: 0022-5347
eISSN: 1527-3792
期刊信息
目录： Current Contents Connect®
其他信息
IDS 号: AI0IS
Web of Science 核心合集中的 "引用的参考文献": 27
Web of Science 核心合集中的 "被引频次": 0
影响因子 4.36 4.064
2014 5 年
JCR® 类别 类别中的排序 JCR 分区
UROLOGY & NEPHROLOGY 10/78 Q1
数据来自第 2014 版 Journal Citation Reports®
引文网络
0 被引频次
27 引用的参考文献
查看 Related Records
查看引证关系图
创建引文跟踪
(数据来自 Web of ScienceTM 核心合集)
全部被引频次计数
0 / 所有数据库
0 / Web of Science 核心合集
0 / BIOSIS Citation Index
0 / 中国科学引文数据库
0 / Data Citation Index
0 / Russian Science Citation Index
0 / SciELO Citation Index
使用次数
最近 180 天: 2
2013 年至今: 11
进一步了解
此记录来自:
Web of ScienceTM 核心合集
在其他数据库中查看记录:
查看 最新数据 (在 Current Contents Connect® 中)
查看 生物学数据 (在 BIOSIS Citation IndexSM 中)
建议修正
如果希望提高此记录中数据的质量，请提供修正建议。
期刊信息
目录： Current Contents Connect® |
|
回复此楼
材料与化工 322求调剂
+4