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Çó·ÒëÏÂÃæÕâ¾ä»° Fluorescent in situ hybridisation Sample handling, fixing and staining was performed as described in Bassin et al. (Bassin et al., 2011a). Probes used to stain all bacteria consisted out of a mixture of EUB338, EUB338II and EUB338III (Amann et al., 1990; Daims et al., 1999). To stain the PAOs present, a mixture of PAO462, PAO651 and PAO846 described by Crocetti et al. (2000) was used. Probes GAOQ431 and GAOQ989 were used to stain the glycogen accumulating organisms (GAO) population (Crocetti R et al., 2002). The samples were examined with a Zeiss Axioplan 2 epifluorescence microscope equipped with filter set 26 (bp 575e625/FT645/bp 660e710), 20 (bp 546/12/ FT560/bp 575e640), 17 (bp 485/20/FT 510/bp 5515e565) for Cy5, Cy3 and fluos respectively. |
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