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The anaerobic digestion tests were conducted based on the well-established biochemical methane potential (BMP) procedure, as described in the guideline VDI 4630 (VDI, 2006). In detail, the batch fermentations were carried out in 100 mL glass syringes according to the Hohenheimer Biogas Yield Test (HBT) (Helffrich and Oechsner, 2003). To ensure anaerobic conditions and to collect the biogas, the syringes were gas tightened by using mediumviscosity silicone paste (GE Bayer Silicones GmbH & Co KG, Erkrath,
Germany). Three way valves allowed sampling of the biogas. For stirring and heating, the syringes were placed in a continuously rotating vertical disc (5 rpm) located in a 42 C heating chamber.


VDI, 2006. Fermentation of Organic Materials ¨C Characterisation of the Substrate, Sampling, Collection of Material Data, Fermentation Tests. Beuth Verlag, Berlin.

Helffrich, D., Oechsner, H., 2003. Hohenheimer biogas yield test ¨C comparing various laboratory methods on biomass fermentation. Landtechnik 58, 148¨C 149.
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