24小时热门版块排行榜    

北京石油化工学院2026年研究生招生接收调剂公告
查看: 197  |  回复: 0

yyy309

新虫 (初入文坛)

[求助] 注射器发酵装置

最近在文献中看到了一种简易的BMP测定方法,利用100mL注射器作为发酵装置,想求教使用过这种方法的前辈一些问题:
我看的文献中提到了使了100mL注射器、30mL基质、顶部三通阀、硅胶密封、恒温振荡器,
那么在实际进行中有什么需要注意的地方么(包括实验过程以及发酵装置的细节)?
对于注射器有什么要求么?
对于基质(主要是基质的产期能力)有没有限制?

谢谢大家!

下面是文献中的方法,引用的参考文献都是书、下不到
The anaerobic digestion tests were conducted based on the well-established biochemical methane potential (BMP) procedure, as described in the guideline VDI 4630 (VDI, 2006). In detail, the batch fermentations were carried out in 100 mL glass syringes according to the Hohenheimer Biogas Yield Test (HBT) (Helffrich and Oechsner, 2003). To ensure anaerobic conditions and to collect the biogas, the syringes were gas tightened by using mediumviscosity silicone paste (GE Bayer Silicones GmbH & Co KG, Erkrath,
Germany). Three way valves allowed sampling of the biogas. For stirring and heating, the syringes were placed in a continuously rotating vertical disc (5 rpm) located in a 42 C heating chamber.


VDI, 2006. Fermentation of Organic Materials – Characterisation of the Substrate, Sampling, Collection of Material Data, Fermentation Tests. Beuth Verlag, Berlin.

Helffrich, D., Oechsner, H., 2003. Hohenheimer biogas yield test – comparing various laboratory methods on biomass fermentation. Landtechnik 58, 148– 149.
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 yyy309 的主题更新
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 0703调剂 +7 拾玖壹 2026-04-04 8/400 2026-04-04 20:56 by imissbao
[考研] 考研调剂 +4 四川王涛 2026-04-04 4/200 2026-04-04 20:11 by wxiongid
[考研] 331求调剂 +3 niby 2026-04-02 3/150 2026-04-04 19:56 by 蓝云思雨
[考研] 363求调剂 +7 zh096 2026-04-04 7/350 2026-04-04 17:13 by dongzh2009
[考研] 一志愿华中农业大学0710(A)初试329分 求调剂 +3 一名26考研生 2026-04-04 3/150 2026-04-04 17:10 by dongzh2009
[考研] 283分求调剂 +5 试试看呗 2026-04-04 5/250 2026-04-04 17:06 by dongzh2009
[考研] 一志愿0817化学工程与技术,求调剂 +24 我不是只因 2026-04-02 28/1400 2026-04-04 15:15 by dongzh2009
[考研] 271分求调剂学校 +11 zph158488! 2026-04-02 11/550 2026-04-04 01:06 by userper
[考研] 本科985,专业0812分336求调剂 +4 莫莫很行 2026-04-03 4/200 2026-04-03 21:31 by zhq0425
[考研] 建环,能源,土木老师路过看一看!!! +5 嘿嘿uu 2026-04-01 5/250 2026-04-03 11:47 by znian
[考研] 抱歉 +5 田洪有 2026-03-30 5/250 2026-04-03 10:24 by linyelide
[考研] 一志愿陕西师范大学生物学317分 +5 1563日。 2026-04-02 5/250 2026-04-03 06:58 by ilovexiaobin
[考研] 293求调剂 +4 珂珂乐 2026-04-02 4/200 2026-04-02 20:10 by 6781022
[考研] 413求调剂 +3 柯某某 2026-03-31 3/150 2026-04-02 16:59 by zzsw+
[考研] 材料调剂 +11 一样YWY 2026-03-31 11/550 2026-04-01 22:25 by zhouyuwinner
[考研] 求调剂0703 +5 周嘉尧 2026-03-31 8/400 2026-04-01 20:32 by ltltkkk
[考研] 调剂 +3 好好读书。 2026-04-01 3/150 2026-04-01 17:06 by zhouyuwinner
[考研] 335求调剂 +3 321* 2026-03-31 4/200 2026-04-01 00:00 by 321*
[考研] 本科211安全工程,初试290分,求调剂 +3 2719846834 2026-03-28 3/150 2026-03-31 13:52 by 热情沙漠
[考研] 274求调剂 +6 xiao爱同学 2026-03-30 6/300 2026-03-31 10:04 by cal0306
信息提示
请填处理意见