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summer晴

新虫 (初入文坛)

[求助] 纤维素酶测量方法

在测量纤维素酶的过程中,文献介绍”One unit of activity is defined as the amount of the enzyme required to release 1 umol reducing groups per min per g of original fresh weight of berry flesh.“ 但是文献并没有介绍具体公式,请问这个计算公式是什么,以及”D-glucose as the standard“是不是需要标准曲线的意思?希望各位大神能帮忙解答下
原文如下:
“Cellulase activity was determined by measuring the reducing groups released from carboxymethyl cellulose (Chin, Ali, & Lazan, 1999). The formation of reducing groups was estimated against D-glucose as the standard. The reaction mixture contained 0.1 ml of crude enzyme, 0.4 ml of 1% (w/v) carboxymethyl cellulose, and 0.5 ml 0.1 M sodium acetate buffer, pH 5.0. Incubation was carried out at 37 C for 1 h followed by addition of DNS. In control tubes the substrate was added after the heat treatment. After heating in a boiling water bath for 5 min, the absorbance was measured at 540 nm. One unit of activity is defined as the amount of the enzyme required to release 1 umol reducing groups per min per g of original fresh weight of berry flesh.“
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wkuiy07

铜虫 (小有名气)

你直接查一下DNS法,就知道了,生化实验中都有

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3楼2016-06-05 23:43:04
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wkuiy07

铜虫 (小有名气)

DNS法肯定需要做标准曲线的,有的文献会提示你依次为对照的

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2楼2016-06-05 23:42:25
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晃晃飞天

新虫 (正式写手)

4楼2016-08-04 21:56:34
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