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【答案】应助回帖
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vxiaoxue: 金币+10, 翻译EPI+1, ★★★很有帮助, 非常感谢。。。。。。 2016-03-14 19:22:47
Purpose: This review etablished respiratory syncytial virus (RSV) infection in young BALB / c mice RSV pneumonia model by inanasal method.It exam content the changes of the serum IL-2 by using the method of ELISA and detected NF kappa bp65 expression changes with the immunohistochemical technique.
By meams of the obseration that mulberry leaves on RSV induced by treatment of pneumonia in young mice model ,we can provide the experimental basis for the clinical treatment of RSV pneumonia.
Materials and methods:
Materials and methods:
1. The intranasal inoculation of respiratory syncytial virus long line of young mice RSV infection model and general state of diet, respiratory, skin, hair and body weight changes, lung index determination and lung tissue pathological light microscope observation results to identified mice RSV pneumonia model
2. 120 young BALB / c mice were randomly divided into normal group, model group, treatment group, control group, a total of 4 groups, with 30 rats in each group, except the normal group, other groups were established model of RSV infection. Mice infected with RSV 2h, the treatment group treated with mulberry leaves 91mg/ (kg - D) by gavage. The control group was treated with ribavirin 114mg/ (kg-d) and the normal group and model group were given the same volume of saline by gavage, 1 times / D, respectively, the 3,5,7 day after modeling, killed, with 10 rats in each group observed the preventive and therapeutic effects of mulberry leaves on RSV pneumonia in mice.
3 the expression of B kappa p65 NF- in different time points of lung tissue was detected by immunohistochemical method. The expression of IL-2 in different time points in each group was detected by ELISA method.
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