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目 的：本研究通过滴鼻方法建立呼吸道合胞病毒（RSV）感染幼龄BALB/c小鼠RSV肺炎模型，采用ELISA法，检测血清中IL-2的含量变化，采用免疫组化法，检测肺组织中、NF-κBp65表达的变化，观察桑叶对RSV诱导幼龄小鼠肺炎模型的治疗效果，为临床治疗RSV肺炎提供实验依据。 材料与方法： 1.采用鼻腔接种呼吸道合胞病毒 long 株建立幼龄小鼠 RSV 感染模型，通过饮食、呼吸、皮毛、活动量及体重等一般状态的改变，肺指数测定及肺组织病理光镜下观察结果鉴定小鼠RSV肺炎模型。 2.将120只幼龄BALB/c小鼠随机分为正常组、模型组、治疗组、对照组，共4组，每组30只，除正常组外，其余组均建立RSV感染模型。小鼠感染RSV病毒2h后，治疗组予桑叶91mg/(kg•d)灌胃，对照组组予病毒唑114mg/(kg•d)灌胃，正常组与模型组予等体积生理盐水灌胃, 1次/d，分别于造模后第3、5、7天处死取材，每组10只，观察桑叶对小鼠RSV肺炎的防治作用。 3.采用免疫组织化学方法，检测各组小鼠肺组织不同时间点 NF-κB p65表达情况；采用ELISA方法，检测各组小鼠血清不同时间点IL-2表达情况。 |
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vxiaoxue: 金币+10, 翻译EPI+1, ★★★很有帮助, 非常感谢。。。。。。 2016-03-14 19:22:47
vxiaoxue: 金币+10, 翻译EPI+1, ★★★很有帮助, 非常感谢。。。。。。 2016-03-14 19:22:47
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Purpose: This review etablished respiratory syncytial virus (RSV) infection in young BALB / c mice RSV pneumonia model by inanasal method.It exam content the changes of the serum IL-2 by using the method of ELISA and detected NF kappa bp65 expression changes with the immunohistochemical technique. By meams of the obseration that mulberry leaves on RSV induced by treatment of pneumonia in young mice model ,we can provide the experimental basis for the clinical treatment of RSV pneumonia. Materials and methods: Materials and methods: 1. The intranasal inoculation of respiratory syncytial virus long line of young mice RSV infection model and general state of diet, respiratory, skin, hair and body weight changes, lung index determination and lung tissue pathological light microscope observation results to identified mice RSV pneumonia model 2. 120 young BALB / c mice were randomly divided into normal group, model group, treatment group, control group, a total of 4 groups, with 30 rats in each group, except the normal group, other groups were established model of RSV infection. Mice infected with RSV 2h, the treatment group treated with mulberry leaves 91mg/ (kg - D) by gavage. The control group was treated with ribavirin 114mg/ (kg-d) and the normal group and model group were given the same volume of saline by gavage, 1 times / D, respectively, the 3,5,7 day after modeling, killed, with 10 rats in each group observed the preventive and therapeutic effects of mulberry leaves on RSV pneumonia in mice. 3 the expression of B kappa p65 NF- in different time points of lung tissue was detected by immunohistochemical method. The expression of IL-2 in different time points in each group was detected by ELISA method. 初略翻译，你自己看着再修改修改！！！！ |
2楼2016-03-14 14:40:06
