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泪雨花舞

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[求助] 求问如何配制含4.5M MgCl2的PBS和含0.5M NaCl的PBS

刚才在文献中看到两种PBS,PBS containing 0.5 M NaCl 和PBS containing 4.5 M MgCl2,请教各位应该如何配制这两种PBS,谢谢。

原文奉上:
For purification of Stx2e, cell extract from 1 L of culture was
applied onto a 2-ml volume of D-galactose gel or TAR column
equilibrated with PBS containing 0.5 M NaCl. After washing the
column, bound protein was eluted with PBS containing 4.5 M
MgCl2, and 2 ml of each fraction was collected. The eluent was
pooled and dialyzed against PBS overnight, and the
precipitated products were removed by centrifugation.
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