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分类	共搜索到 30 个相关话题(最多显示前5000个)	作者	最后发表

文献求助	Weak promoters to drive selection marker expression:... LucieGrindes1,CamilleFlorimond2,SébastienRibault2,CélineRaymond2,WilfridDieryck3,Weakpromoterstodriveselectionmarkerexpression:...K1cellsJBiotechnol.2023Jun10:369:43-54.10.1016/...	知音	2025-05-20 06:01
硕博家园	GST融合蛋白为何用WB就是检测不到？本人用pGEX-6p-1载体构建的原核表达载体，在BL21(DE3)菌中优化表达后，跑SDS-PAGE，结果显示表达与预期...蛋白marker红色的是70kDa，第2孔是空载体诱导的GST蛋白，后面8个孔是优化表达的样品。...	洛克2014	2019-04-05 02:01
绿色求助(高悬赏)	Production of chimeric pigs and the ...using mitochondrial de mitochondrialdeoxyribonucleicacidasacellmarker.info'.2.'][info3]BiolReprodinfo'.3.'][info4]1994info'.4.'][info5]51(6):1069-75info'.5.'][info7]...	格调虾米	2017-11-23 03:08
文献求助	求助Expert Rev Mol Diagn.文献1篇 [info1]SalviS1,MartignanoF1,MolinariC1,GurioliG1,CalistriD1,DeGiorgiU2,ConteducaV2,Casadio...urinecellfreeDNAasamarkerforcancer.info'.2.'][info3]ExpertRevMol...	yuntaishan2008	2017-11-19 03:57
文献求助	Higher Red Meat Intake May Be a Marker of Risk,Not a Risk ... [info1]ErlonOliveiradeAbreuSilva,MD;AlineMarcadenti,MScinfo'.1.'][info2]HigherRedMeatIntakeMayBeaMarkerofRisk,NotaRiskFactorItselfinfo'.2.'][info3]Arch...	wenli0509	2017-10-30 07:20
文献求助	The Preparation of Colloidal Gold ...Marker in Electron Mic [info1]J.DeMey,M.Moeremansinfo'.1.'][info2]ThePreparationofColloidalGoldProbesandTheirUseasMarkerinElectronMicroscopyinfo'.2.'][info3]AdvancedTechniquesin...	白建慧	2017-06-28 11:43
文献求助	求助文献一篇！ SlaterG,WeissAandBursteinDEinfo'.1.'][info2]GLUT1glucosetransporterexpressionincolorectalcarcinoma:Amarkerforpoorprognosis.info'.2.'][info3]Cancerinfo'.3....	18886014593	2017-01-03 01:31
文献求助	Detection of molecular markers linked to Ry...germplasm for marker DeKoeyer,DMurphy,Ainfo'.1.'][info2]DetectionofmolecularmarkerslinkedtoRygenesinpotatogermplasmformarker-assistedselectionforextremeresistancetoPVYinAAFC...	Bosha	2016-12-15 08:21
文献求助	高分求助SCI论文一篇 possibleearlymarkerofaprothromboticstateduringthefirsttrimester...[info7]https://th.schattauer.de/en/contents/archive/issue/special/manuscript/26063.htmlinfo'.7.']	沧海行云	2016-08-26 12:31
文献求助	Cytokine-Independent Detection of Antigen-Specific Germinal ... TorrentsdelaPe?aA4,KasturiSP5,DanJM6,BothwellM7,SandersRW4,PulendranB3,SilvestriG3,CrottyS8...ImmunizedNonhumanPrimatesUsingaLiveCellActivation-InducedMarker...	geraldine	2016-07-27 03:50
文献求助	急用，谢谢 M.info'.1.']...	0745324	2016-06-20 02:51
分子生物	构建的重组菌无法诱导表达出目的蛋白我有一个900bp的基因，最先是和pET28a连接，导进BL21(DE3)，一开始是表达的，但是纯化之后发现有背景蛋白，即没有诱导的菌也能表达出一个和目的片段大小相等的蛋白，...P.S：line1：marker；...	CarolChan	2016-06-13 07:24
文献求助	求助文献一篇 [info1]AlTmimiL,VandeVeldeM,MeynsB,MeurisB,SergeantP,MilisenK,PottelH,PoesenK,RexS.info'.1.'][info2]SerumproteinS100asmarkerofpostoperativedeliriumafter...	gracegao1991	2016-06-07 04:35
文献求助	万方里就有，谢谢 DeDeyn；FJ,Bosker；PGM,Luiten；JA,denBoer；RC,OudeVoshaarinfo'.1.'][info2]Neutrophilgelatinase-associatedlipocalin:anovelinflammatorymarkerassociatedwithlate...	liuguofei	2016-02-17 03:26
文献求助	求助文献一篇~ trituberculatus:DeNovoAssembly,Growth-RelatedGeneIdentificationandMarkerDiscoveryinfo'.2.'][info3]PLoSOneinfo'.3.'][info4]2014;info'.4.'][info5]9(4):e94055info...	Alex_ppz	2016-01-28 02:20
文献求助	CD27:marker and mediator of T-cell activation? [info1]HintzenRQ,deJongR,LensSM,vanLierRA.info'.1.'][info2]CD27:markerandmediatorofT-cellactivation?info'.2.'][info3]ImmunolToday.info'.3.'][info4]1994Jul;...	shaojunyang	2016-01-22 03:32
硕博家园	DNA ladder实验阶梯怎么不明显呢 ladder实验，做了好久，效果一直不好，这两张图片算是效果好点的，但这种算是阶梯出来了吗，为什么对照组（marker右边第一条）看起来也有阶梯状呢，收集...45v-2h-1%胶	蓝白de	2016-01-19 02:43
文献求助	Critical role for BRCA1 expression as a marker of ... [info1]DeLucaP1,DeSierviA2.info'.1.'][info2]CriticalroleforBRCA1expressionasamarkerofchemosensitivityresponseandprognosis.info'.2.'][info3]FrontBiosci...	xmczhoumei	2016-01-03 07:41
文献求助	based identification of stable hazelnut ...tryptic marker pep DeMeulenaer,B;Devreese,Binfo'.1.'][info2]MALDI-basedidentificationofstablehazelnutproteinderivedtrypticmarkerpeptidesinfo'.2.'][info3]FOODADDITIVESAND...	沉默的琴弦	2015-12-23 07:35
文献求助	求助一篇文献~ fluorescentmarkerandquantifyproteinexpressionatthecellularlevel.[发自手机版http://emuch.net/3g][info1]PierreBarbierdeReuilleAffiliatedwith...	gaojin0411	2015-11-18 08:57
文献求助	求Journal of Crohn's and Colitis论文1篇 [info1]MagalideBruyn,IngridArijs,GertDeHertogh,MarcFerrante,GertVanAssche,PaulRutgeerts,Sé...Metalloproteinase-9ComplexasaSurrogateMarkerforMucosalHealingin...	paopao81	2015-09-21 12:45
文献求助	De Novo Assembled Transcriptome Analysis and SSR Marker ... Y.-P.Xiainfo'.1.'][info2]DeNovoAssembledTranscriptomeAnalysisandSSRMarkerDevelopmentofaMixtureofSixTissuesfromLiliumOrientalHybrid‘Sorbonne’info'.2.']...	wh6125	2015-07-16 05:29
分子生物	pet28a 和pet32a 连接701bp的目的片段后跑电泳图有杂带，求分析转化至DH5α中，菌液PCR有条带，提质粒后已送测序，等待结果中，将质粒又转化至BL21（DE3）中，挑8个单克隆做菌液PCR有...Lane2-5菌液PCR正确701bp（含有pet28a-701菌液中），Lane6：没加样；...	95846713	2015-01-05 01:22
论文翻译求助完结子版	Avian-Induced Pluripotent Stem Cells Derived Using Human ... betweeneachpopulationwith2-tailedPvaluesigni?cant.EmbryoidbodyformationanddifferentiationEmbryoidbodies(EBs)wereformedbyplating2.0·106qiPSCsinmTeSR1...	AAXMCH	2014-05-14 11:15
硕博家园	求助BL21（DE3）表达外源基因的问题最近在用BL21（DE3）表达一段1800bp左右的外源基因，蛋白预计大小73kD，加上His-Tag等大概83kD，载体PET30a，IPTG诱导条件试了0.1mM，1mM，10mM，温度试...但是大小很奇怪，第一张图（marker：...	jingjinglea	2014-04-10 02:58