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[求助]
DNA序列上传NCBI问题求解
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在NCBI上传动物COI序列后收到NCBI如下邮件，望有经验前辈赐教，该如何解决。本人比较外行，DNA提取扩增后都是由测序公司测序拼接的，对基因序列中存在的问题也不太清楚。NCBI邮件如下： We have received the following 57 sequence submissions from you: SUB7027792 : (57) Some or all of the protein coding sequences contain reading frame shifts (insertions/deletions based on BLAST similarity search results and/or an alignment), and/or have translations that show little or no similarity to other proteins in the database. This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's Provide a corrected submission file through the Submission Portal: |
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