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The histological studies on impacted limbs showed surface lesions on both
plateaus, thickening of the underlying subchondral bone at 12 months and numerous occult
microcracks at the calcified cartilage–subchondral bone interface at 6 and 12 months, without
significant changes in cartilage thickness or its mechanical properties versus controls.
Yet, there was an
increase in both the matrix and fiber moduli and a decrease in the permeability of uncovered, medial
plateau cartilage in both limbs of impacted animals between 6 and 12 months post impact that was not
documented in control animals
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