| 查看: 822 | 回复: 3 | ||
[求助]
帮忙查询引用次数 已有1人参与
|
|
Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions. PLoS One. 2012 谁帮忙在web of science 上帮忙查询下 |
回复此楼» 猜你喜欢
有多少人是今天查系统知道结果的？
已经有17人回复
-
国自然面上复盘~欢迎讨论
已经有6人回复
-
售SCI一区文章，我:8.O.551.O.5.4,科目全,可伽急
已经有4人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.54,科目齐全,可+急
已经有4人回复
-
基金不中，共勉
已经有5人回复
-
看板上这么多中的，有点像50人群里49个人都是骗子的那种感觉……
已经有3人回复
-
我不理解！
已经有23人回复
-
2026年的国家社科基金项目通讯评审的新规则与新动向、新挑战
已经有10人回复
-
系统查不到
已经有10人回复
-
怎么查啊
已经有5人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- SCI投稿过程总结、投稿状态解析、拒稿后对策及接受后期相关问答
已经有142人回复
- 求帮忙用wos查询一篇论文的引用情况，需要截图 - 着急，在线等
已经有1人回复
- 求帮忙用wos查询一篇论文的引用情况，需要截图 - 项目申请用，谢谢
已经有2人回复
- 求助：帮忙查询2篇论文在web of science上的引用次数
已经有7人回复
- SCI论文写作的立意与经验技巧
已经有2人回复
- 如何查询某本教材的被引用次数
已经有0人回复
liouzhan654
版主 (知名作家)
- 应助: 798 (博后)
- 贵宾: 5.504
- 金币: 53654.6
- 散金: 5660
- 红花: 182
- 沙发: 8
- 帖子: 9431
- 在线: 2139.6小时
- 虫号: 2048939
- 注册: 2012-10-08
- 性别: GG
- 专业: 传热传质学
- 管辖: 论文投稿
【答案】应助回帖
★ ★ ★ ★ ★
感谢参与，应助指数 +1
guangyi: 金币+5, 谢谢 2016-02-21 11:14:47
感谢参与，应助指数 +1
guangyi: 金币+5, 谢谢 2016-02-21 11:14:47
|
目前被引用次数为0次 Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions 作者:Hu, YB (Hu, Yangbo)[ 1 ] ; Feng, LP (Feng, Lipeng)[ 1,2 ] ; Li, YL (Li, Yunlong)[ 1,2 ] ; Zhang, Y (Zhang, Yong)[ 1 ] ; Lu, P (Lu, Pei)[ 1 ] ; Rayner, S (Rayner, Simon)[ 1 ] ; Chen, SY (Chen, Shiyun)[ 1 ] 查看 ResearcherID 和 ORCID PLOS ONE 卷: 7 期: 11 文献号: e50142 DOI: 10.1371/journal.pone.0050142 出版年: NOV 21 2012 查看期刊信息 摘要 Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved. In this study, we have developed a ribosomal binding site (RBS) switching strategy for direct cloning of PCR fragments. RBS is an A/G rich region upstream of the translational start codon and is essential for gene expression. Change from A/G to T/C in the RBS blocks its activity and thereby abolishes gene expression. Based on this property, we introduced an inactive RBS upstream of a selectable marker gene, and designed a fragment insertion site within this inactive RBS. Forward and reverse insertions of specifically tailed fragments will respectively form an active and inactive RBS, thus all background from vector self-ligation and fragment reverse insertions will be eliminated due to the non-expression of the marker gene. The effectiveness of our strategy for TA cloning and blunt end ligation are confirmed. Application of this strategy to gene over-expression, a bacterial two-hybrid system, a bacterial one-hybrid system, and promoter bank construction are also verified. The advantages of this simple procedure, together with its low cost and high efficiency, makes our strategy extremely useful in HTP cloning constructions. 关键词 KeyWords Plus  OSITIVE-SELECTION VECTORS; PCR PRODUCTS; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; IN-VIVO; GENE; RECOMBINATION; SEQUENCE作者信息 通讯作者地址: Chen, SY (通讯作者) 显示增强组织信息的名称 Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Peoples R China. 地址: 显示增强组织信息的名称 [ 1 ] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Peoples R China 显示增强组织信息的名称 [ 2 ] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 电子邮件地址:sychen@wh.iov.cn 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 31170133 National Transgenic Program of China 2011ZX08009-003-001-003 查看基金资助信息 出版商 PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA 类别 / 分类 研究方向:Science & Technology - Other Topics Web of Science 类别:Multidisciplinary Sciences 文献信息 文献类型:Article 语种:English 入藏号: WOS:000311821000189 PubMed ID: 23185557 ISSN: 1932-6203 期刊信息 Impact Factor (影响因子): Journal Citation Reports® 其他信息 IDS 号: 047ER Web of Science 核心合集中的 "引用的参考文献": 30 Web of Science 核心合集中的 "被引频次": 0 |
2楼2016-02-21 11:11:37
zhouqi0903
木虫 (著名写手)
- 应助: 58 (初中生)
- 金币: 3392.9
- 散金: 1179
- 红花: 23
- 帖子: 1102
- 在线: 349.1小时
- 虫号: 2885610
- 注册: 2013-12-21
- 性别: GG
- 专业: 园林学
3楼2016-02-21 11:48:27
liouzhan654
版主 (知名作家)
- 应助: 798 (博后)
- 贵宾: 5.504
- 金币: 53654.6
- 散金: 5660
- 红花: 182
- 沙发: 8
- 帖子: 9431
- 在线: 2139.6小时
- 虫号: 2048939
- 注册: 2012-10-08
- 性别: GG
- 专业: 传热传质学
- 管辖: 论文投稿
4楼2016-02-21 14:03:10
