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Crystals were grown at 18 °C by the hanging2drop method with 10 g/ L of protein , 0. 05 mol/ L HEPES buffer (pH 7. 5) and 35 % MPD in 8 μl drops equilibrated against 0. 5 ml solution of 0. 1 mol/ L HEPES buffer (pH 7. 5) and 70 % MPD. The data were collected with a Mar345 research area detector using a single crystal. The data were scaled and merged using the HKL suite[15 ] . |
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2楼2008-10-12 10:46:34
