| 查看: 469 | 回复: 5 | |||
| 当前主题已经存档。 | |||
| 【悬赏金币】回答本帖问题，作者lirongrong将赠送您 10 个金币 | |||
lirongrong银虫 (小有名气)
|
[求助]
求一篇外文文献【有效期至2008年10月5日】
|
||
|
McClean, K. H., M. K. Winson, L. Fish, A. Taylor, S. R. Chhabra, M. Camara, M. Daykin, J. H. Lamb, S. Swift, B. W. Bycroft, G. S. A. B. Stewart, and P. Williams. 1997. Quorum sensing and Chromobacterium violaceumChromobacterium violaceum: exploitation of violacein production and inhibition for the detection of NN-acylhomoserine lactones. Microbiology 143:3703-3711 [ Last edited by lirongrong on 2008-10-6 at 22:56 ] |
回复此楼» 猜你喜欢
310求调剂
已经有12人回复
-
290求调剂
已经有7人回复
-
电气专硕320求调剂
已经有5人回复
-
一志愿西北工业大学289 085602
已经有33人回复
-
一志愿哈工大 085600 277 12材科基求调剂
已经有24人回复
-
268分085602化学工程调剂
已经有28人回复
-
化学工程调剂289
已经有50人回复
-
求调剂，262机械专硕
已经有8人回复
-
305求调剂
已经有6人回复
-
327求调剂
已经有5人回复
2楼2008-10-04 08:09:01
3楼2008-10-04 08:22:36
|
只有摘要 Quorum sensing relies upon the interaction of a diffusible signal molecule with a transcriptional activator protein to couple gene expression with cell population density. In Gram-negative bacteria, such signal molecules are usually N-acylhomoserine lactones (AHLs) which differ in the structure of their N-acyl side chains. Chromobacterium violaceum, a Gram-negative bacterium commonly found in soil and water, produces the characteristic purple pigment violacein. Previously the authors described a violacein-negative, mini-Tn5 mutant of C. violaceum (CV026) in which pigment production can be restored by incubation with supernatants from the wild-type strain. To develop this mutant as a general biosensor for AHLs, the natural C. violaceum AHL molecule was first chemically characterized. By using solvent extraction, HPLC and mass spectrometry, a single AHL, N-hexanoyl-L-homoserine lactone (HHL), was identified in wild-type C. violaceum culture supernatants which was absent from CV026. Since the production of violacein constitutes a simple assay for the detection of AHLs, we explored the ability of CV026 to respond to a series of synthetic AHL and N-acylhomocysteine thiolactone (AHT) analogues. In CV026, violacein is inducible by all the AHL and AHT compounds evaluated with N-acyl side chains from C4 to C8 in length, with varying degrees of sensitivity. Although AHL compounds with N-acyl side chains from C10 to C14 are unable to induce violacein production, if an activating AHL (e.g. HHL) is incorporated into the agar, these long-chain AHLs can be detected by their ability to inhibit violacein production. The versatility of CV026 in facilitating detection of AHL mixtures extracted from culture supernatants and separated by thin-layer chromatography is also demonstrated. These simple bioassays employing CV026 thus greatly extend the ability to detect a wide spectrum of AHL signal molecules. |
4楼2008-10-04 09:32:56
lirongrong
银虫 (小有名气)
- 博学EPI: 1
- 应助: 0 (幼儿园)
- 金币: 441.3
- 散金: 5
- 帖子: 280
- 在线: 25.9小时
- 虫号: 246690
- 注册: 2006-04-30
- 性别: MM
- 专业: 病原细菌与放线菌生物学
5楼2008-10-04 18:22:08
qiuqiu8120
金虫 (小有名气)
- 应助: 1 (幼儿园)
- 金币: 943.6
- 散金: 47
- 红花: 3
- 帖子: 256
- 在线: 137.5小时
- 虫号: 577989
- 注册: 2008-06-27
- 专业: 凝聚态物性 II ：电子结构
6楼2008-10-05 09:47:00
