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专业: 生物化学

[求助] 细胞生物学题目

求助下面这道题的答案，用中文回答就可以，万分感谢，急用，谢谢！
you are studying the secretory pathway in budding yeast using a combination of genetic, biochemical and cell biological techniques.
(1)you are interested in performinga screen for temperature sensitive secretory mutants in budding yeast. what property of cells blocked in the secretory system will you exploit in this screen? how will you use this property to isolate mutants?
(2)you isolate 5 classes of mutants in this screen. these classes are listed below, along with the location where proteins accumulate.describe what function is defective in each class, and briefly explain your answer.
class A-Cytoplasm
class B-Endoplasmic Reticulum
class C-ER to Golgi Vessicles
class D-Golgi
class E-Secretory Vessicles
(3)what protein might be mutated in Class A mutants? what protein might be mutanted in Class B mutants?
(4)you isolate a mutant in which proteins that are normally targeted to the endosome instead accumulate in the Golgi. what protein might defective in these mutants? what other process might also be affected in these mutants?

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