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At low salt concentration, the surfactant had a higher affinity with the water phase (Winsor I system) and produced O/W emulsions of a density lower than that of water. Thus, a cream layer atop the excess water was formed (see white phase in the test tubes). Above the transition concentration (or the so-called optimum salinity), the affinity of the surfactant with the oil was higher compared to that with water (Winsor II), and W/O emulsions were formed, with a density higher than that of the oil phase and thus, they settled in the bottom of the test tubes. These observations are evident for both salinity scans, after gentle and strong emulsification.
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