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组织培养VC的使用方法
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组织培养培养基里防褐化时需要加VC,请问大家知道VC的使用方法吗？ 发自小木虫Android客户端 |
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Plant cell reuirements for vitamin concentration vary according to the plant species and type of culture. But pre-treatment with Vitamin E at 40% is recommended to achieve the lowest browning and highest callus induction results in date palm cultures. In addition, cold pre-treatment for three days at 5 degrees Celsiu is recommended when the using of antioxidant is not preferred. As for Vitamin C (Ascorbic acid), 15% Ascorbic acid and 10% Citric acid, respectively, gave lower results compared with Vitamin E treatments. This reslut is in harmony with the findings of Taha et. al (2009) who stated that treated explants with 150mg/L citric acid and 100mg/L ascorbic acid prior to surface sterilization treatment, did not gave satisfied resluts for reducing browning mango explants. |
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2016-01-08 22:13:38, 305.4 K
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