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snake

金虫 (小有名气)

土豆切成小块煮沸半小时后,直接过滤得到液体就可以了,不用那么麻烦
11楼2016-01-08 16:47:37
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陆封肆闭

至尊木虫 (著名写手)

煮透了即可,可以用玻璃棒扎一下试试,能轻轻一扎就透的就行了,继续煮就成泥了,不要死记说煮多少分钟,没有统一规定,因为没人知道你的火有多大。另外煮土豆的时候要搅拌,否则会糊锅的。
12楼2016-01-08 16:51:43
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反应链

新虫 (正式写手)

1. Preparing Potato Extract

Place potatoes in a saucepan with approximately 1.2 liters of tap water and boil on a stove for 1 hour.

Remove saucepan from heat and let liquid cool until it can be comfortably handled (about 10 minutes).

Pour liquid potato extract through a strainer or cheesecloth and collect it in another container.

2. Preparing the Medium

Add dextrose and agar to 1 liter of the potato extract water.

Test the pH of the solution with pH test paper or a pH meter.

If the pH is above 6.5, adjust it down by adding one drop of the hydrochloric acid solution at a time and retesting.

If the pH is below 6, adjust it up by adding one drop of the sodium hydroxide solution at a time and retesting.

3. Sterilization

Thoroughly mix the medium and divide it into smaller batches of 250 mL poured into the four 500-mL flasks (Note: each flask must be no more than half full to avoid boil over).

Cover each flask with a piece of loosely crimped aluminum foil, so the foil will not fall off, but does not make a tight seal.

Place the flasks in a pressure cooker.

Operate the pressure cooker in accordance with the manufacturer's instructions to sterilize the PDA at 121 degrees C (250 degrees F) at a pressure of 103,421 Pascals(15 PSI) for 15 minutes.

Shut off the pressure cooker and let it return to atmospheric pressure, then remove the flasks and let them cool until they can be handled.

4. Pouring the Medium

Line up the sterile petri dishes on a flat surface.

Once the flasks have cooled to handling temperature, but before the medium begins to solidify, grasp a flask with one hand and remove and set aside its foil lid with the other hand.

For each petri dish, lift the lid with one hand, quickly fill halfway with liquid PDA, and replace the lid.

Repeat Step 3 above until all petri dished are filled.

If there is any PDA left over, loosely recover the flask with its original foil lid, let it cool and solidify, tighten the foil lid and store in a refrigerator.

References: http://www.ehow.com/how_5872701_make-potato-dextrose-agar.html
13楼2016-01-08 17:32:08
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740317257

新虫 (小有名气)

煮的时间不够。半个小时差不多应该成泥了。

发自小木虫Android客户端
14楼2016-01-09 08:46:58
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我是Cindy呀

新虫 (初入文坛)

加了土豆,再加硫酸铜为什么会显蓝色,而把等量硫酸铜加入清水中却不显蓝色,是不是土豆中某种成分和硫酸铜结合了?这有多大的影响呢?求答复

发自小木虫IOS客户端
15楼2018-04-20 07:46:47
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陆封肆闭

至尊木虫 (著名写手)

16楼2018-04-20 14:26:23
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