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[求助]
PDA培养基配制 已有7人参与
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| 配制PDA培养基时，土豆切成小块煮沸半小时后，土豆并没有成土豆泥。各位怎么做的，是直接过滤得到液体；还是把煮软的小块搅拌成泥，再过滤得到液体呢？ |
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1. Preparing Potato Extract Place potatoes in a saucepan with approximately 1.2 liters of tap water and boil on a stove for 1 hour. Remove saucepan from heat and let liquid cool until it can be comfortably handled (about 10 minutes). Pour liquid potato extract through a strainer or cheesecloth and collect it in another container. 2. Preparing the Medium Add dextrose and agar to 1 liter of the potato extract water. Test the pH of the solution with pH test paper or a pH meter. If the pH is above 6.5, adjust it down by adding one drop of the hydrochloric acid solution at a time and retesting. If the pH is below 6, adjust it up by adding one drop of the sodium hydroxide solution at a time and retesting. 3. Sterilization Thoroughly mix the medium and divide it into smaller batches of 250 mL poured into the four 500-mL flasks (Note: each flask must be no more than half full to avoid boil over). Cover each flask with a piece of loosely crimped aluminum foil, so the foil will not fall off, but does not make a tight seal. Place the flasks in a pressure cooker. Operate the pressure cooker in accordance with the manufacturer's instructions to sterilize the PDA at 121 degrees C (250 degrees F) at a pressure of 103,421 Pascals(15 PSI) for 15 minutes. Shut off the pressure cooker and let it return to atmospheric pressure, then remove the flasks and let them cool until they can be handled. 4. Pouring the Medium Line up the sterile petri dishes on a flat surface. Once the flasks have cooled to handling temperature, but before the medium begins to solidify, grasp a flask with one hand and remove and set aside its foil lid with the other hand. For each petri dish, lift the lid with one hand, quickly fill halfway with liquid PDA, and replace the lid. Repeat Step 3 above until all petri dished are filled. If there is any PDA left over, loosely recover the flask with its original foil lid, let it cool and solidify, tighten the foil lid and store in a refrigerator. References: http://www.ehow.com/how_5872701_make-potato-dextrose-agar.html |
13楼2016-01-08 17:32:08
张香玲1017
铁杆木虫 (著名写手)
冷夜雨
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2楼2016-01-05 17:28:36
【答案】应助回帖
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感谢参与，应助指数 +1
CC-Brandy: 金币+2, ★★★很有帮助 2016-01-06 14:00:29
感谢参与，应助指数 +1
CC-Brandy: 金币+2, ★★★很有帮助 2016-01-06 14:00:29
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切块成1立方厘米左右大小，沸水煮20分钟。直接过滤得滤液。加葡萄糖就好了。 发自小木虫Android客户端 |
3楼2016-01-05 23:54:25
4楼2016-01-06 22:36:42