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三分饿的吧至尊木虫 (著名写手)
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[求助]
生化与分子生物学的一篇英文文献摘要
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| A direct competitive enzyme-linked immunosorbent assay (ELISA) for triazophos was developed, which was based on the THHe monoclonal antibody (McAb) and a heterologous enzyme tracer (THBu-HRP). The influence of several physicochemical factors (temperature, time, pH, salt, detergent, and solvent) on the immunoassay was studied. For the standard curve, an I50 of 0.21 microg/L and a limit of detection (I20) of 0.02 microg/L was obtained in a high salt concentration buffer (0.05 M PBS, pH 6.0) with 0.05% BSA, which means an almost 3-fold improvement in the assay sensitivity in comparison with the nonoptimized conditions. The optimized ELISA has been used to quantify triazophos in water and soil samples spiked at different amounts. The excellent recoveries achieved confirmed the potential of the immunoassay for environmental monitoring of triazophos in waters and soils without purification steps. |
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baoshanqiu
至尊木虫 (著名写手)
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三分饿的吧: 金币+120, 翻译EPI+1, ★★★★★最佳答案 2016-01-06 09:29:34
三分饿的吧: 金币+120, 翻译EPI+1, ★★★★★最佳答案 2016-01-06 09:29:34
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The influence of several physicochemical factors (temperature, time, pH, salt, detergent, and solvent) on the immunoassay was studied. For the standard curve, an I50 of 0.21 microg/L and a limit of detection (I20) of 0.02 microg/L was obtained in a high salt concentration buffer (0.05 M PBS, pH 6.0) with 0.05% BSA, which means an almost 3-fold improvement in the assay sensitivity in comparison with the nonoptimized conditions. The optimized ELISA has been used to quantify triazophos in water and soil samples spiked at different amounts. The excellent recoveries achieved confirmed the potential of the immunoassay for environmental monitoring of triazophos in waters and soils without purification steps. 采用THHe单克隆抗体(McAb)和一种非均相酶示踪剂(THBu-HRP)建立了检测三唑磷的直接竞争性酶联免疫吸附试验(ELISA)。探讨了几种理化因素（温度、时间、pH、盐、去垢剂和溶剂）对免疫检测的影响。在含0.05% BSA高盐浓度缓冲液(0.05 M PBS, pH 6.0)条件下，得到标准曲线的I50为0.21 microg/L，检测限(I20) 为0.02 microg/L，意即较之于非优化条件，其检测灵敏度提高近3倍。优化的ELISA已经用于定量检测峰值不同的水和土壤样品中得三唑磷。得到的良好回收率确认了运用免疫检测法于环境监测水和土壤中的三唑磷而无需纯化过程的潜力。 |
2楼2016-01-05 23:19:12
