| 查看: 642 | 回复: 1 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
三分饿的吧至尊木虫 (著名写手)
|
[求助]
求一篇英文摘要翻译
|
||
| Two haptens of the insecticide triazophos (O,O-diethyl O-[1-phenyl-1H-1,2,4-triazol-3-yl] phosphorothioate) were synthesized by introducing appropriate spacers in the O-ethyl site of the analyte molecular structure. First, thiophosphoryl chloride (PSCl3) reacts with methanol at low temperature to give O-ethyl dichlorothiophosphate. After reacting with 1-phenyl-3H-1,2,4-triazol, the O-ethyl dichlorothiophosphate was transformed into the intermediate O-ethyl O-(1-phenyl-1H-1,2,4-triazol-3-yl) chlorothiophosphate. Then the intermediate reacts with 4-aminobutyric acid and 6-aminobutyric acid to produce hapten I and hapten II, respectively. The molecule structures of the two haptens were identified by H-1 nuclear magnetic resonance spectrum and mass spectrum. An enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody was also developed to evaluate the two haptens. Results showed that the monoclonal antibodies with high titers were obtained after immunizing with protein conjugates of these haptens and that the immunoassay has high affinity and specificity to triazophos. These results suggested that the haptens were synthesized successfully and could be used for immunoassay for the rapid screening and sensitive determination of this insecticide. |
回复此楼» 猜你喜欢
酰胺脱乙酰基
已经有11人回复
-
同年申请2项不同项目，第1个项目里不写第2个项目的信息，可以吗
已经有4人回复
-
有时候真觉得大城市人没有县城人甚至个体户幸福
已经有10人回复
-
天津大学招2026.09的博士生，欢迎大家推荐交流（博导是本人）
已经有5人回复
-
有院领导为了换新车，用横向课题经费买了俩车
已经有5人回复
-
CSC & MSCA 博洛尼亚大学能源材料课题组博士/博士后招生｜MSCA经费充足、排名优
已经有6人回复
-
面上项目申报
已经有3人回复
-
博士延得我，科研能力直往上蹿
已经有7人回复
-
面上基金申报没有其他的参与者成吗
已经有5人回复
-
遇见不省心的家人很难过
已经有22人回复
baoshanqiu
至尊木虫 (著名写手)
- 翻译EPI: 204
- 应助: 103 (高中生)
- 金币: 15293.5
- 红花: 41
- 帖子: 1611
- 在线: 431.6小时
- 虫号: 3856202
- 注册: 2015-05-08
- 性别: GG
- 专业: 胃肠道免疫相关疾病
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
三分饿的吧: 金币+130, 翻译EPI+1, ★★★★★最佳答案 2016-01-06 09:23:27
三分饿的吧: 金币+130, 翻译EPI+1, ★★★★★最佳答案 2016-01-06 09:23:27
| 通过在分析物分子结构中的乙酰基位点引入适当的空隙合成了杀虫剂三唑磷（邻，邻-二乙酰邻-[1-苯基-1氢-1,2,4-三唑-3-基]磷硫酰）的两种半抗原。首先，硫代磷酰氯(PSCl3)与甲醇在低温反应得到邻-乙酰二氯硫磷酸。后者与1-1-苯基-3氢-1,2,4-三唑反应后转化成中间产物邻-乙酰邻-（1-苯基-1氢-1,2,4-三唑-3-基）氯硫磷酸。该中间产物再与4-氨基丁酸和6-氨基丁酸反应分别生成半抗原 I 和半抗原II。两种半抗原的分子结构经过H-1核磁共振谱和质谱鉴定。也建立了基于单克隆抗体的酶联免疫吸附试验(ELISA)来评价这两种半抗原。结果表明免疫这些半抗原的蛋白结合物后得到高滴度的单克隆抗体，免疫检测对三唑磷具有高亲和力和特异性。这些结果说明半抗原的成功合成，可用于快速筛选和灵敏测定这种杀虫剂的免疫检测。 |
2楼2016-01-06 00:06:45