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三分饿的吧至尊木虫 (著名写手)
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[求助]
求一篇英文摘要翻译
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| Two haptens of the insecticide triazophos (O,O-diethyl O-[1-phenyl-1H-1,2,4-triazol-3-yl] phosphorothioate) were synthesized by introducing appropriate spacers in the O-ethyl site of the analyte molecular structure. First, thiophosphoryl chloride (PSCl3) reacts with methanol at low temperature to give O-ethyl dichlorothiophosphate. After reacting with 1-phenyl-3H-1,2,4-triazol, the O-ethyl dichlorothiophosphate was transformed into the intermediate O-ethyl O-(1-phenyl-1H-1,2,4-triazol-3-yl) chlorothiophosphate. Then the intermediate reacts with 4-aminobutyric acid and 6-aminobutyric acid to produce hapten I and hapten II, respectively. The molecule structures of the two haptens were identified by H-1 nuclear magnetic resonance spectrum and mass spectrum. An enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody was also developed to evaluate the two haptens. Results showed that the monoclonal antibodies with high titers were obtained after immunizing with protein conjugates of these haptens and that the immunoassay has high affinity and specificity to triazophos. These results suggested that the haptens were synthesized successfully and could be used for immunoassay for the rapid screening and sensitive determination of this insecticide. |
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baoshanqiu
至尊木虫 (著名写手)
- 翻译EPI: 204
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- 专业: 胃肠道免疫相关疾病
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三分饿的吧: 金币+130, 翻译EPI+1, ★★★★★最佳答案 2016-01-06 09:23:27
三分饿的吧: 金币+130, 翻译EPI+1, ★★★★★最佳答案 2016-01-06 09:23:27
| 通过在分析物分子结构中的乙酰基位点引入适当的空隙合成了杀虫剂三唑磷（邻，邻-二乙酰邻-[1-苯基-1氢-1,2,4-三唑-3-基]磷硫酰）的两种半抗原。首先，硫代磷酰氯(PSCl3)与甲醇在低温反应得到邻-乙酰二氯硫磷酸。后者与1-1-苯基-3氢-1,2,4-三唑反应后转化成中间产物邻-乙酰邻-（1-苯基-1氢-1,2,4-三唑-3-基）氯硫磷酸。该中间产物再与4-氨基丁酸和6-氨基丁酸反应分别生成半抗原 I 和半抗原II。两种半抗原的分子结构经过H-1核磁共振谱和质谱鉴定。也建立了基于单克隆抗体的酶联免疫吸附试验(ELISA)来评价这两种半抗原。结果表明免疫这些半抗原的蛋白结合物后得到高滴度的单克隆抗体，免疫检测对三唑磷具有高亲和力和特异性。这些结果说明半抗原的成功合成，可用于快速筛选和灵敏测定这种杀虫剂的免疫检测。 |
2楼2016-01-06 00:06:45